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MABS279

Sigma-Aldrich

Anti-GPR30, clone 2F2 Antibody

clone 2F2, from mouse

Synonym(s):

G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1, FEG-1, G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor, LYGPR

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2F2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GPER1(2852)

General description

GPR30, also known as G-protein coupled estrogen receptor 1, Chemoattractant receptor-like 2, Flow-induced endothelial G-protein coupled receptor 1 (FEG-1), G-protein coupled receptor 30, GPCR-BR, IL8-related receptor DRY12, Lymphocyte-derived G-protein coupled receptor (LYGPR), or Membrane estrogen receptor (mER), and encoded by the gene GPER/CEPR/CMKRL2/DRY12/GPR30,is the common receptor for estrogen. GPR30 binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol (3,4,5)-trisphosphate in the nucleus, and therefore GPR30 is part of the rapid non-genomic signaling events widely observed when cells interact with estrogen. GPR30 is a cell membrane bound protein found in the Golgi apparatus, endoplasmic reticulum and plasma membrane. GPR30 is ubiquitously expressed, but is most abundant in placenta. In brain regions, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GPR30.

Application

Detect GPR30 using this Anti-GPR30 antibody, clone 2F2 validated for use in IHC & IP.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected GPR30 in HEK293 cell lysate (Filardo, E., et al. (2007). Endocrinology. 148(7):3236-3245.).
Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Quality

Evaluated by Immunohistochemistry in human placenta tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GPR30 in human placenta tissues.

Target description

42 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1Īŗ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8Ā°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a productā€™s label following the words ā€˜Lotā€™ or ā€˜Batchā€™.

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George Notas et al.
International journal of molecular sciences, 22(14) (2021-07-25)
Inflammation is important for the initiation and progression of breast cancer. We have previously reported that in monocytes, estrogen regulates TLR4/NFĪŗB-mediated inflammation via the interaction of the ErĪ± isoform ERĪ±36 with GPER1. We therefore investigated whether a similar mechanism is

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