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MABS341

Sigma-Aldrich

Anti-LRP6 Antibody, clone 1C10

clone 1C10, from mouse

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Synonym(s):
Low-density lipoprotein receptor-related protein 6, LRP-6
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1C10, monoclonal

species reactivity

human, rat, mouse

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... LRP6(4040)

General description

Low density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.

Immunogen

Flag-tagged recombinant protein corresponding to human LRP6.

Application

Immunoprecipitation Analysis: A representative lot immunoprecipitated LRP6 in certain cell lyates (Khan, Z., et al. (2007). Mol Cell Biol. 27(20):7291-7301.).
This LRP6 antibody is validated for use in WB & IP for the detection of the LRP6 protein.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LRP6 in 10 µg of HEK293 cell lysate. For enhanced results, it is recommended for end-users to perform higher dilutions of this antibody.

Target description

~200 kDa observed

Physical form

Format: Purified
Purified mouse monoclonal IgG containing PBS with 0.05% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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