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MABS436

Sigma-Aldrich

Anti-ATE1 Antibody, clone 6F11

clone 6F11, from rat

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Synonym(s):
Arginyl-tRNA--protein transferase 1, Arginyltransferase 1, R-transferase 1, Arginine-tRNA--protein transferase 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6F11, monoclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATE1(11101)

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vibrant-m

MABS436

Anti-ATE1 Antibody, clone 6F11

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

6F11, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, mouse

species reactivity

human

species reactivity

rabbit, horse, dog, rat, guinea pig, human

species reactivity

mouse, rat, human

biological source

rat

biological source

rabbit

biological source

rabbit

biological source

rabbit

NCBI accession no.

NP_038827

NCBI accession no.

-

NCBI accession no.

NP_005162

NCBI accession no.

P18846

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General description

ATE1 plays an important role a specialized ubiquitin mediated protein degradation pathway. ATE1 is involved in the post-translational conjugation of arginine to the N-terminal or mid-chain Aspartate or Glutamate amino acids of proteins. The arginylation is required for effective ubiquitin mediated protein degradation. ATE1 is a central player in the Arg/N-end rule pathway of ubiquitin mediated protein degradation. This system disposes of malformed or misdirected proteins and protein fragments. ATE1 has two isoforms, isoform 1 is nuclear localized and isoform 2 is cytoplasmically localized. ATE1 is widely expressed, and recent research demonstrates that ATE1 mediated arginylation is essential for platelet myosin phosphorylation, clot retraction and thrombosis formation. Recent research also shows that the Arg/N-end rule pathway and ATE1 may play a role in helping to prevent neurodegeneration caused by the buildup of protein fragments found in various neurological disease states such as Alzheimer′s disease, amyotrophic lateral sclerosis, and Parkinson′s disease.

Immunogen

Recombinant protein corresponding to mouse ATE1.

Application

Research Category
Signaling
Research Sub Category
Signaling Neuroscience
This Anti-ATE1 Antibody, clone 6F11 is validated for use in Western Blotting and Immunocytochemistry for the detection of ATE1.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected ATE1 in 10 µg of mouse liver tissue lysate.
Western Blotting Analysis: A representative lot detected ATE1 in ATE1 isoforms and mouse liver extract tissue lysate (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).
Immunocytochemistry Analysis: A representative lot detected ATE1 in immunostaining and cell extract preparation, immortalized mouse embryonic fibroblasts derived from wild-type and Ate1 cells (Wang, J., et al. (2011). Chemistry & Biology. 18:121-130).

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ATE1 in 10 µg of HepG2 cell lysate.

Target description

~60 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Marie E Fina et al.
Scientific reports, 11(1), 9376-9376 (2021-05-02)
Regulator of G-protein signaling 7 (RGS7) is predominately present in the nervous system and is essential for neuronal signaling involving G-proteins. Prior studies in cultured cells showed that RGS7 is regulated via proteasomal degradation, however no protein is known to
Junling Wang et al.
Methods in enzymology, 626, 89-113 (2019-10-14)
Protein arginylation-enzymatic addition of the amino acid arginine (Arg) to proteins, mediated by arginyltransferase ATE1, has been discovered in 1963, but is still relatively poorly understood. Studies of arginylation present many technical challenges, which arise from the fact that Arg
Iuliia Pavlyk et al.
Traffic (Copenhagen, Denmark), 19(4), 263-272 (2018-02-01)
β-actin plays key roles in cell migration. Our previous work demonstrated that β-actin in migratory non-muscle cells is N-terminally arginylated and that this arginylation is required for normal lamellipodia extension. Here, we examined the function of β-actin arginylation in cell
Junling Wang et al.
Developmental biology, 430(1), 41-51 (2017-08-29)
Arginylation is an emerging protein modification mediated by arginyltransferase ATE1, shown to regulate embryogenesis and actin cytoskeleton, however its functions in different physiological systems are not well understood. Here we analyzed the role of ATE1 in brain development and neuronal
Michael D Birnbaum et al.
Oncogene, 38(6), 838-851 (2018-09-05)
Most prostate cancer cases remain indolent for long periods of time, but metastatic progression quickly worsens the prognosis and leads to mortality. However, little is known about what promotes the metastasis of prostate cancer and there is a lack of

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