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MABS835

Sigma-Aldrich

Anti-EGFR Antibody, clone 7F12.1

clone 7F12.1, from mouse

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Synonym(s):
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7F12.1, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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MABS835

Anti-EGFR Antibody, clone 7F12.1

clone

7F12.1, monoclonal

clone

polyclonal

clone

225, monoclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

human

species reactivity

human, rat, mouse

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

isotype

IgG2bκ

isotype

-

isotype

IgG1κ

isotype

-

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General description

Epidermal growth factor receptor (EC 2.7.10.1; UniProt P00533; also known as Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1, NISBD2) gene (Gene ID 1956) in human. The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands. Upon ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains, which allows its interaction with adaptor proteins and induction of several signaling pathways including PI3K-AKT; PLC-gamma-PKC; Notch1; and Ras-Raf. Hence, it contributes to a diverse range of cellular processes, including cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

Specificity

Epitope region is present in spliced isoform 1 (p170), but not in isoform 2 (p60, Truncated, TEGFR), isoform 3 (p110) or isoform 4 reported by UniProt (P00533).

Immunogen

GST-tagged recombinant human EGFR fragment correspoding to a cytoplasmic region C-terminal to the kinase domain.

Application

Detect EGFR using this mouse monoclonal , Cat. No. MABS835, validated for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized A431 cells.

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected plasma membrane EGFR immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized A431 cells.
Research Category
Signaling

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected EGFR in 10 µg of A431 cell lysate.

Target description

~170 kDa observed. Target band size appears larger than the calculated molecualar weight of 134.3 kDa due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: 04-338

Physical form

Format: Purified
Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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