MilliporeSigma
All Photos(2)

Documents

MABT366

Sigma-Aldrich

Anti-Claudin-1/CLDN1 Antibody, clone 7A5

clone 7A5, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Claudin-1, Senescence-associated epithelial membrane protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7A5, monoclonal

species reactivity

human

should not react with

mouse

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

Related Categories

General description

Claudin-1 (UniProt O95832; also known as Senescence-associated epithelial membrane protein) is encoded by the CLDN1 (also known as CLD1, ILVASC, NISCH, SEMP1) gene (ORF UNQ481/PRO944; Gene ID 9076) in human. Tight junctions (TJs) are highly specialized membrane barriers that block the free passage of small molecules, microorganisms, and cells across the paracellular space, as well as provide a physical barrier between the apical and basolateral membrane domains of a polarized epithelial cell. Claudins are TJ proteins whose main function is to regulate the passage of ions across the paracellular space. There exist at least 27 human claudins with differential epithelial expression patterns, allowing tissue-specific paracellular transport of ions. Claudin-1 is expressed on apical and basolateral surfaces of hepatocytes in normal liver tissue. Claudin-1 is a known host factor for hepatitis C virus (HCV) entry via interaction with CD81 and cell-to-cell HCV transmission. Claudin-1 is a four-transmembrane (a.a. 8-28, 82-102, 116-136, 164-184) protein, having both its N- and C-terminal ends exposed cytoplasmically (a.a. 1-7 & 185-211).

Specificity

Clone 7A5 specifically recognized human claudin-1/CLDN1 by targeting an epitope in the second extracellular loop and prevented claudin-1-dependent hepatitis C virus (HCV) entry into host cells. Clone 7A5 does not react with human claudin-2, -4, -5, -6, -7, or -9, nor mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).

Immunogen

Epitope: Second extracellular loop.
Recombinant human Claudin-1/CLDN1.

Application

Flow Cytometry Analysis: 5.0 µg/mL from a representative lot immunostained HT1080 cells expressing exogenously transfected human claudin-1/CLDN1 and Huh-7.5.1 human hepatoma cells, but not mock-transfected HT1080 cells or non-claudin-1-expressing S7-d6 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously expressed claudin-1 in CLDN1-transfected, but not mock-transfected, HT1080 cells (Courtesy of Professor Masuo Kondoh, PhD, Osaka University, Japan).
Immunocytochemistry Analysis: A representative lot immunostained the surface of Huh-7.5.1 human hepatoma cells, but not the non-claudin-1-/CLDN1-expressing S7-A cells (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Flow Cytometry Analysis: A representative lot specifically immunostained HT1080 cells expressing exogenously transfected human claudin-1 (CLDN1), but not HT1080 cells expressing human claudin-2, -4, -5, -6, -7, or -9, nor L cells expressing mouse claudin-1 (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Flow Cytometry Analysis: A representative lot immunostained HEK293T transfectants expressing FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in human EL2 abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Western Blotting Analysis: A representative lot detected FLAG-tagged human claudin-1/CLDN1 and human-mouse claudin-1 chimeras with the second human extracellular loop (EL2), but not chimeras with the mouse EL2. M152L, but not V155I, mutation in the second human extracellular loop abolished the immunoreactivity (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
ELISA Analysis: A representative lot detected claudin-1/CLDN1 immunoreactivity in 3.7% formaldehyde-fixed Huh-7.5.1 human hepatoma cells by "cell ELISA" (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Neutralization Analysis: A representative lot inhibited HCV infection of cultured Huh-7.5.1 human hepatoma cells in vitro and of human liver-chimeric mice in vivo (Fukasawa, M., et al. (2015). J. Virol. 89(9):4866-4879).
Research Category
Cell Structure
Research Sub Category
Infectious Diseases - Viral
This Anti-Claudin-1 Antibody, clone 7A5 is validated for use in Immunocytochemistry, Western Blotting, Flow Cytometry for the detection of CLDN1.

Quality

Evaluated by Immunocytochemistry in HepG2 cells.

Immunocytochemistry Analysis: 10 µg/mL of this antibody detected Claudin-1/CLDN1 in HepG2 cells.

Target description

~20 kDa observed. 22.74 kDa calculated.

Physical form

Format: Purified
Protein G purified.
Purified mouse monoclonal IgG1κ antibody in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service