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MABT376

Sigma-Aldrich

Anti-Ero1 Antibody, clone 2G4/12

clone 2G4/12, from mouse

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Synonym(s):
ERO1-like protein alpha, ERO1-L, ERO1-L-alpha, Endoplasmic oxidoreductin-1-like protein, Oxidoreductin-1-L-alpha
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2G4/12, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ERO1L(30001)

General description

ERO1-like protein alpha (ERO1) is also called endoplasmic oxidoreductin-1-like protein and Oxidoreductin-1-L-alpha. ERO1 is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1 is stimulated by hypoxia, indicating that it is regulated by the HIF-pathway. ERO1 may be responsible for much of the reactive oxygen species (ROS) in the cell, and therefore is a source of oxidative stress. EROS1 is required for the folding of immunoglobulin proteins, responsible for the release of the unfolded cholera toxin, and plays an important role in ER stress-induced, CHOP-dependent apoptosis. ERO1 is widely expressed at low levels, with high levels in upper digestive tract.

Immunogen

GST-tagged recombinant protein corresponding to Human Ero1.

Application

Anti-Ero1 Antibody, clone 2G4/12 is a highly specific mouse monoclonal antibody, that targets ERO1-like protein alpha & has been tested in western blotting, IP & Immunofluorescence.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: 1 µg/mL from a representative lot detected Ero1 from 10 µg of the following cell lysates--A431, HEK293, HeLa, HepG2, human placenta tissue, HUVEC, jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7.

Immunoprecipitation Analysis: A representative lot immunoprecipitated Ero1 in Ero1 transfected HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Immunofluorescence Analysis: A representative lot detected Ero1 in HeLa cell lysate (Prof. R. Sitia, San Rafaele Scientific Institute, Milano, Italy).

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Ero1 in 10 µg of HEK293 cell lysate.

Target description

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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