MilliporeSigma
All Photos(1)

Documents

MABT845

Sigma-Aldrich

Anti-Cytokeratin-20 Antibody, clone XQ1

clone XQ1, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Keratin, type I cytoskeletal 20, CK-20, Keratin-20, K20
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

XQ1, monoclonal

species reactivity

mouse

should not react with

human

packaging

antibody small pack of 25 μg

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... KRT20(54474)
mouse ... Krt20(66809)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABT1344MABT913SAB4502249
biological source

mouse

biological source

rabbit

biological source

rat

biological source

rabbit

species reactivity

mouse

species reactivity

human

species reactivity

mouse, human

species reactivity

human, rat, mouse

clone

XQ1, monoclonal

clone

polyclonal

clone

TROMA-3, monoclonal

clone

polyclonal

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

UniProt accession no.

Q9D312

UniProt accession no.

P35900

UniProt accession no.

P19001

UniProt accession no.

P35900

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Keratin, type I cytoskeletal 20 (UniProt: Q9D312; also known as Cytokeratin-20, CK-20, Keratin-20, K20) is encoded by the Krt20 gene (Gene ID: 66809) in murine species. Cytokeratin-20 is type I keratin of the intermediate filament family and is recognized as a marker of intestinal differentiation. It is expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Cytokeratin-20 is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. Cytokeratin-20 can undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 235 by caspases. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447).

Specificity

Clone XQ1 is a mouse monoclonal antibody that detects cytoketatin-20 in murine tissues.

Immunogen

A full-length purified murine Cytokeratin-20.

Application

Anti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunofluorescence Analysis: A representative lot detected Cytokeratin-20 in Immunofluorescence applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).

Western Blotting Analysis: A representative lot detected Cytokeratin-20 in Western Blotting applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).
Research Category
Cell Structure

Quality

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Cytokeratin-20 in mouse colon tissue sections.

Target description

49.03 kDa calculated.

Physical form

Format: Purified
Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service