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MHA000T2B

Millipore

Tryptic Soy Broth

pkg of 2 mL, ampoule non-selective medium used to detect total heterotrophic microorganisms in water and other liquids

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Synonym(s):
TSB, CASO, Casein Soybean Digest Broth, Tryptone Soya Broth, SCDB
eCl@ss:
32160801
NACRES:
NA.74

Quality Level

form

liquid

shelf life

12 mo.

feature

ready-to-use

packaging

pkg of 2 mL
pack of 50 tests

technique(s)

cell counting: suitable (total viable count (TVC))

color

light amber

input

sample type bottled water
sample type cosmetics
sample type beverage(s)
sample type food(s)
sample type wine
sample type sport drink
sample type cider
sample type beer
sample type soft drink

pH

7.3±0.2 (25.0 °C)

application(s)

clinical
cosmetics
environmental
food and beverages
industrial qc
microbiology

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure monitor
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

nonselective for

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This Item
220921.463161.46317
Tryptic Soy Broth pkg of 2 mL, ampoule non-selective medium used to detect total heterotrophic microorganisms in water and other liquids

Millipore

MHA000T2B

Tryptic Soy Broth

Tryptic Soy Broth EP, USP, bag of 10 L (with MPC connector and triple-bagged)

Millipore

1.46316

Tryptic Soy Broth

Tryptic Soy Broth bottle of, ready-to-use, bottle volume 100 mL , filling volume

Millipore

1.46317

Tryptic Soy Broth

shelf life

12 mo.

shelf life

-

shelf life

-

shelf life

12 mo.

feature

ready-to-use

feature

-

feature

ready-to-use

feature

closure type crimp cap with septum, ready-to-use

packaging

pkg of 2 mL, pack of 50 tests

packaging

bottle of 500 g

packaging

bag of 10 L (with MPC connector and triple-bagged)

packaging

bottle of

technique(s)

cell counting: suitable (total viable count (TVC))

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

-

pH

7.3±0.2 (25.0 °C)

pH

-

pH

7.3 ( in H2O)

pH

7.3

General description

Tryptic Soy Broth is a general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for the enrichment and cultivation of aerobic and facultatively anaerobic microorganisms that are not excessively fastidious.{18} In clinical microbiology, it may be used for the suspension, enrichment and cultivation of strains isolated on other media. In clinical microbiology, the medium is used in a variety of procedures, e.g., for the preparation of the inoculum and for suspending strains for Kirby-Bauer disc diffusion susceptibility testing and for the microbiological test procedure of culture media. Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Glucose (=dextrose) is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH. Colonies appear clear to white colonies; some may produce pigment.

Application

Tryptone Soy Broth is used as a non-selective medium used to detect total heterotrophic microorganisms in water, dairy products and other liquids like Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Packaging

2 mL plastic ampoules

Specifications

Incubation Temperature: 25–35 °C
Incubation Time: 48–72 hrs

Culture Medium

Trypticase Soy

Analysis Note

Clear to white colonies; some may produce pigment.
QC organisms
S. epidermidis (ATCC 14990)

C. albicans (ATCC 10231)

E. coli (ATCC 25922)

B. subtilis (ATCC 6633)

A.brasilliensisformerly A. niger (ATCC 16404 / WDCM 00053)

P. aeruginosa (ATCC 27853)
Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

Slide 1 of 4

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Diagnostic microbiology
Forbe BA et al.
Bailey and Scott's Diagnostic Microbiology, 11 (1988)
Media for Isolation-Identification-Cultivation-Maintenance of Medical Bacteria
MacFaddin JF
Williams and Wilkins (1985)

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