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Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

liquid, clone Y13-259, Calbiochem®

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biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies


Y13-259, monoclonal




≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse



storage condition

do not freeze



shipped in

wet ice

storage temp.


target post-translational modification


General description

Purified rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Recognizes the ~21 kDa Ras protein (see comments).
Recognizes both phosphorylated and nonphosphorylated forms of v-H-Ras, and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Also recognizes H, K, and N-Ras human translated products.
This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections, Neutralization Studies for the detection of v-H-Ras (Ab-1).


Epitope: within amino acids 62-76
recombinant p21 Ras


Frozen Sections (5 µg/ml)

Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence)

Immunofluorescence (2.5 µg/ml)

Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample)

Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)

Neutralization Studies (see application references)


Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
SW480 or Y1 cells or normal skin tissue

Other Notes

Broadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids




Not applicable


Not applicable

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