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SCC129

Sigma-Aldrich

HT-22 Mouse Hippocampal Neuronal Cell Line

HT-22 mouse neuronal cell line is a valuable cell model for studies of glutamate-induced toxicity in neuronal cells.

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Synonym(s):
HT22 Cell Line
eCl@ss:
32011203

biological source

mouse

Quality Level

technique(s)

cell based assay: suitable

shipped in

dry ice

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SCC116SCC048SCC222
technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | stem cell: suitable

technique(s)

cell based assay: suitable, cell culture | mammalian: suitable

shipped in

dry ice

shipped in

ambient

shipped in

liquid nitrogen

shipped in

liquid nitrogen

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Glutamate is a major excitatory neurotransmitter used as a signaling molecule between nerve cells and is involved in most aspects of normal brain function including cognition, learning and memory. Various glutamate receptors are found throughout the brain and include NMDA, AMPA/kainate and metabotropic receptors (mGluR). Normal glutamate levels are essential for normal brain function, however high levels may result in over-excitation of nerve cells, leading to eventual cell damage and/or cell death. Glutamate-induced cytotoxicity has been implicated in neurodegenerative disorders such as Alzheimer’s disease, Huntington’s disease and Parkinson’s disease along with other conditions including spinal cord injury and multiple sclerosis. Therapies that may inhibit or reduce the effects of glutamate activity are thus of great therapeutic interest.
HT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line . The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen . HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.

Cell Line Description

Neural Lineage Cells

Application

EMD Millipore has a limited license to sell this product for research use only, and not for any commercial uses. Excluded commercial uses include without limitation manufacturing, providing a service, therapeutic, diagnostic and prophylactic uses, and any other commercial uses. Use of this product by a purchaser for any purpose other than for research is unauthorized and prohibited.

Inquiries about licensing for commercial or other uses should be directed to:

Office of Technology Development
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037
Phone: (858) 453-4100 extension 1278
Email: OTD@salk.edu
Research Category
Neuroscience

Toxicity

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Kuangfu Hsiao et al.
Cell, 183(2), 522-536 (2020-10-01)
Working memory is a form of short-term memory that involves maintaining and updating task-relevant information toward goal-directed pursuits. Classical models posit persistent activity in prefrontal cortex (PFC) as a primary neural correlate, but emerging views suggest additional mechanisms may exist.
Brian K A Choi et al.
PloS one, 14(8), e0220056-e0220056 (2019-08-02)
Abortive cell cycle (ACC) re-entry of apoptotic neurons is a recently characterized phenomenon that occurs after central nervous system (CNS) injury or over the course of CNS disease. Consequently, inhibiting cell cycle progression is neuroprotective in numerous CNS pathology models.
J B Davis et al.
Brain research, 652(1), 169-173 (1994-07-25)
A neuronal cell line, HT-22, is sensitive to glutamate cytotoxicity via a non-receptor mediated oxidative pathway. 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, blocks this glutamate-induced cell death. Down-regulation of protein kinase C eliminates the protection against glutamate cytotoxicity
Kyung-Ran Kim et al.
Experimental & molecular medicine, 53(7), 1134-1147 (2021-07-09)
Calbindin, a major Ca2+ buffer in dentate granule cells (GCs), plays a critical role in shaping Ca2+ signals, yet how it regulates neuronal function remains largely unknown. Here, we found that calbindin knockout (CBKO) mice exhibited dentate GC hyperexcitability and
Peng Huang et al.
Annals of translational medicine, 9(20), 1594-1594 (2021-11-19)
Phosphodiesterase 4D (PDE4D) inhibitor is commonly used to treat depression, but side effects seriously decrease its efficacy. PDE4D was a downstream target mRNA of miR-139-5p. Therefore, we examined the effects of hippocampal miR-139-5p gain- and loss-of-function on depression-like behaviors, the

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