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SCC148

Sigma-Aldrich

MB49 Mouse Bladder Carcinoma Cell Line

MB49 mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.

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Synonym(s):
MB-49
eCl@ss:
32011203
NACRES:
NA.81

biological source

mouse

Quality Level

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

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SCC070SCC072SCC173
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

liquid nitrogen

shipped in

liquid nitrogen

shipped in

ambient

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

General description

MB49 cells are derived from C57BL/Icrf-a’ mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture . Transformed cells transplanted into syngeneic mice were shown to generate carcinomas . While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed . This abnormality is a frequent early event in human bladder cancer. A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth . MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone, human chorionic gonadotrophin (hCG) . MB49 cells exhibit low to no expression of MHC-Class I and II molecules . However, upon exposure to IFN-, the expressions of MHC Class I and II are significantly upregulated .

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the "Academic Use Agreement" as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Victoria T Fabris et al.
Cancer genetics, 205(4), 168-176 (2012-05-09)
Bladder cancer is frequently associated with chromosomal abnormalities, and the complexity of karyotypes increases with tumor progression. The murine model MB49 is one of the most widely studied models of bladder cancer. We developed the invasive cell line MB49-I by
Adnan Deronic et al.
Cancer immunology, immunotherapy : CII, 70(12), 3629-3642 (2021-05-06)
Non-responders to checkpoint inhibitors generally have low tumor T cell infiltration and could benefit from immunotherapy that activates dendritic cells, with priming of tumor-reactive T cells as a result. Such therapies may be augmented by providing tumor antigen in the
Yuexia Liang et al.
Theranostics, 12(18), 7729-7744 (2022-12-02)
Rationale: IgA can induce activation of neutrophils which are the most abundant cell type in blood, but the development of IgA as therapeutic has been confounded by its short half-life and a weak ability to recruit NK cells as effector
Shai White-Gilbertson et al.
Bladder (San Francisco, Calif.), 3(1) (2016-03-22)
The MB49 syngeneic, murine model of bladder cancer has been widely used for more than 35 years. In humans, bladder cancer is one third as prevalent in women as in men, with a trend toward lower prevalence in parous compared
I C Summerhayes et al.
Journal of the National Cancer Institute, 62(4), 1017-1023 (1979-04-01)
Neoplastic transformation of C57BL/lcrf-a' mouse bladder epithelium was induced in long-term primary cultures by a single 24-hour treatment with 7,12-dimethylbenz[a]anthracene on day 2 of culture. Transformed foci appeared earlier (40--60 days) and at a higher frequency (28%) in cultures from

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