MilliporeSigma
All Photos(1)

Documents

10102105001

Roche

Adenosine Deaminase (ADA)

from calf intestine

Sign Into View Organizational & Contract Pricing

Synonym(s):
ADA
Enzyme Commission number:

biological source

bovine (calf) intestine

Quality Level

form

solution

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

mol wt

230-440 kDa (high)
30-47 kDa (low )

packaging

pkg of 2 mL (10 mg)

manufacturer/tradename

Roche

optimum pH

7.0-7.4

shipped in

wet ice

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
101021210011011043400110108014001
vibrant-m

10102105001

Adenosine Deaminase (ADA)

vibrant-m

10102121001

Adenosine Deaminase (ADA)

vibrant-m

10110434001

Xanthine Oxidase (XOD)

vibrant-m

10108014001

Papain

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

specific activity

~200 units/mg protein (At 25 °C with adenosine as the substrate.)

specific activity

~1 units/mg protein (At 25 °C with xanthine as the substrate.)

specific activity

30 U/mg, ~30 units/mg protein (At 25 °C with BAEE as the substrate.)

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

mol wt

230-440 kDa (high), 30-47 kDa (low )

mol wt

-

mol wt

-

mol wt

23 kDa

optimum pH

7.0-7.4

optimum pH

7.0-7.4

optimum pH

8.5-9.0

optimum pH

6.0-7.0

General description

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.

Application

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.

Biochem/physiol Actions

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Preparation Note

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

Adenosine suitable for cell culture, BioReagent

Sigma-Aldrich

A4036

Adenosine

Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service