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10102547001

Roche

AMP-PNP

Adenylyl-imidodiphosphate

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Synonym(s):
Adenosine 5′-(β,γ-imido)triphosphate lithium salt hydrate, β,γ-Imidoadenosine 5′-triphosphate lithium salt hydrate, AMP-PNP, ATP[β,γ-NH], Adenylyl imidodiphosphate lithium salt hydrate, App(NH)p
Empirical Formula (Hill Notation):
C10H17N6O12P3 · xLi+ · yH2O
CAS Number:
Molecular Weight:
506.20 (free acid basis)
Beilstein/REAXYS Number:
6047109
UNSPSC Code:
12352204

Quality Level

form

powder

mol wt

Mr 506.2 (ANP-PNP)
Mr 529.9 (AMP-PNP-Li4)

packaging

pkg of 25 mg

manufacturer/tradename

Roche

SMILES string

[Li+].[Li+].[Li+].[Li+].[H]O[H].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP([O-])(=O)OP([O-])(=O)NP([O-])([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H17N6O12P3/c11-8-5-9(13-2-12-8)16(3-14-5)10-7(18)6(17)4(27-10)1-26-31(24,25)28-30(22,23)15-29(19,20)21/h2-4,6-7,10,17-18H,1H2,(H,24,25)(H2,11,12,13)(H4,15,19,20,21,22,23)/t4-,6-,7-,10-/m1/s1

InChI key

PVKSNHVPLWYQGJ-KQYNXXCUSA-N

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General description

AMP-PNP (adenylyl-imidodiphosphate) is a non-hydrolysable analogue of ATP. It inhibits fast axonal transport and facilitates the interaction between membranous organelles and microtubules.
AMP-PNP(adenylyl-imidodiphosphate) is an artificial substrate of enzyme, adenylate cyclase. Adenylate cyclase helps to hydrolyze adenylyl-imidodiphosphate slowly than ATP.

Application

AMP-PNP has been used in the preparation of nucleotide solutions, two-motor fluorescence assay, preparation of flow-cells with kinesin and cascade binding assays.
AMP-PNP is a competitive inhibitor of most ATP-dependent systems. A good substrate for enzymes hydrolyzing between the α- and β-phosphorus atom, yet is resistant to enzymes cleaving between the β- and γ-phosphorus atom. Substrate of snake venom phosphodiesterase and adenylate cyclase.
AMP-PNP has been used for two-motor fluorescence assay.

Features and Benefits

Contents
88% AMP-PNP (from N), 4.5% lithium, 6% water

Quality

Contaminants: ≤1.5% Pi , <0.1% ATP (enzymatically)

Storage and Stability

Store at -15–-25 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Nucleotide dependence of the dimerization of ATP binding cassette nucleotide binding domains
Fendley GA, et al.
Biochemical and Biophysical Research Communications, 480(2), 268-272 (2016)
Cristina Olivieri et al.
eLife, 9 (2020-04-28)
In the nucleus, the spatiotemporal regulation of the catalytic subunit of cAMP-dependent protein kinase A (PKA-C) is orchestrated by an intrinsically disordered protein kinase inhibitor, PKI, which recruits the CRM1/RanGTP nuclear exporting complex. How the PKA-C/PKI complex assembles and recognizes
Arthur Chow et al.
Methods in enzymology, 667, 365-402 (2022-05-08)
Pseudokinases often operate through functionally related enzymes and receptors. A prime example is the pseudokinase KSR (Kinase Suppressor of RAS), which can act as both an amplifier and inhibitor of members in the RAS-MAPK (Mitogen Activated Protein Kinase) signaling pathway.
High accuracy measurements of nanometer-scale distances between fluorophores at the single-molecule level
Niekamp S, et al.
bioRxiv, 234740-234740 (2017)
Sarah Triclin et al.
Nature materials, 20(6), 883-891 (2021-01-23)
Microtubule instability stems from the low energy of tubulin dimer interactions, which sets the growing polymer close to its disassembly conditions. Molecular motors use ATP hydrolysis to produce mechanical work and move on microtubules. This raises the possibility that the

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