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11426362001

Roche

Hexokinase (HK)

suspension, suitable for detection

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Synonym(s):
HK, Hexokinase
Enzyme Commission number:

form

suspension

Quality Level

specific activity

~450 units/mg protein (At 25 °C and pH 7.6 with D-glucose and ATP as the substrates.)

packaging

pkg of 1 mL (1,500 U)

manufacturer/tradename

Roche

pH range

6.0-8.0

application(s)

detection

shipped in

wet ice

storage temp.

2-8°C

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This Item
H6380SAB1300477SRP6065
Hexokinase (HK) suspension, suitable for detection

11426362001

Hexokinase (HK)

Hexokinase 1 human recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

SRP6065

Hexokinase 1 human

application(s)

detection

application(s)

-

application(s)

-

application(s)

-

form

suspension

form

lyophilized powder

form

buffered aqueous solution

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−70°C

shipped in

wet ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

Quality Level

100

Quality Level

-

Quality Level

-

Quality Level

-

General description

ATP:D-hexose 6-phosphotransferase
Hexokinase consists of 486 amino acids and has a molecular weight of 57,000 D (SDS-PAGE) in citrate/phosphate buffer. It may form dimers under other buffer conditions.
Hexokinase exists in three isozymes, namely type I, type II and type III that differ in subcellular localization, catalytic and regulatory properties. The type I isozyme serves a catabolic function facilitating the entry of glucose into glycolytic metabolism for energy production. The type II and type III enzymes perform anabolic functions, such as generating G6P for glycogen synthesis or metabolism through the phosphogluconate pathway for lipid synthesis. Type I and type II isozymes have been found to be bound to mitochondria. The type III isozyme has been found to have perinuclear localization in several cell types.

Application

Hexokinase (HK) has been used in enzyme assays and chemiluminescence assay. It has also been used to determine ATP concentration.
Use Hexokinase for the determination of D-glucose, D-fructose, and D-sorbitol in food or biological research samples. Hexokinase may also be used for the assay of other saccharides, which are convertible to glucose or fructose, and is, therefore, useful in the assay of many glycosides.
Hexokinase has been used in the enzymatic detection of nitric oxide and in the measurement of ATP by luminescence method.

Biochem/physiol Actions

Hexokinase catalyzes the conversion of hexoses to hexose-6-phosphate in an ATP-dependent manner. It is also called as the glycolytic enzyme as it catalyzes the first step in glucose metabolism with the formation of glucose-6-phosphate (G6P). In eukaryotes, it functions as a glucose sensor.

Quality

Contaminants: <0.002% PGI, <0.0005% G6P-DH and GR, each, <0.001% CK, MK, 6-PGDH, and ADH, each, <0.05% GIDH and invertase (β-fructosidase), each, <30 μg/ml glucose (enzymatically)

Unit Definition

Unit Assay: For measuring hexokinase activity 0.22 M glucose in 0.1 M triethanolamine buffer, pH 7.6, 6.5 mM MgCI2, 2.7 mM ATP, 0.83 mM NADP is incubated in the presence of 1.7 U glucose-6-phosphate dehydrogenase with an appropriate amount of hexokinase (10–20 mU) at +25 °C (total volume: 3.0 ml). The enzyme activity is calculated from the increase of absorbance at 340 nm ( = 6.3 [I × mmol/l-1 × cm-1]) or 365 nm ( = 3.5 [I × mmol/l-1 × cm-1]).

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6.5

Preparation Note

Activator: Hexokinase requires Mg2+ ions (Km = 2.6 mM) for activity. Catecholamines too increase activity.
Stabilizers: Thiols

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Stefan Hof et al.
Frontiers in medicine, 9, 867298-867298 (2022-05-17)
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Zhu L, et al.
American Journal of Physiology. Endocrinology and Metabolism, 306(10) (2014)
J C Jang et al.
The Plant cell, 9(1), 5-19 (1997-01-01)
The mechanisms by which higher plants recognize and respond to sugars are largely unknown. Here, we present evidence that the first enzyme in the hexose assimilation pathway, hexokinase (HXK), acts as a sensor for plant sugar responses. Transgenic Arabidopsis plants
Xu Deng et al.
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The yeast Saccharomyces cerevisiae IMA multigene family encodes four isomaltases sharing high sequence identity from 65% to 99%. Here, we explore their functional diversity, with exhaustive in-vitro characterization of their enzymological and biochemical properties. The four isoenzymes exhibited a preference

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