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11636103001

Roche

PCR Master

(50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase), sufficient for 200 reactions, kit of 1 (2 components), suitable for PCR

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Synonym(s):
PCR

usage

sufficient for 200 reactions
(50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase)

Quality Level

feature

dNTPs included
hotstart: no

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

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This Item
P4600D744012140314001
PCR Master (50 μl final reaction volume each containing 2.5 U Taq DNA-Polymerase), sufficient for 200 reactions, kit of 1 (2 components), suitable for PCR

11636103001

PCR Master

ReadyMix™ Taq PCR Reaction Mix with MgCl2

P4600

ReadyMix Taq PCR Reaction Mix

High Fidelity PCR Master sufficient for ≤200 reactions, kit of 1 (2 components), suitable for PCR

12140314001

High Fidelity PCR Master

usage

sufficient for 200 reactions

usage

sufficient for 100 reactions

usage

sufficient for 100 reactions, sufficient for 20 reactions, sufficient for 400 reactions

usage

sufficient for ≤200 reactions

feature

dNTPs included

feature

dNTPs included, hotstart: no

feature

Multiplex PCR, dNTPs included, hotstart

feature

High Fidelity PCR, dNTPs included, hotstart: no

packaging

kit of 1 (2 components)

packaging

-

packaging

-

packaging

kit of 1 (2 components)

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Roche

input

purified DNA

input

purified DNA

input

purified DNA

input

purified DNA

General description

Routine assays have medium size amplicons and 50% GC content. Taq DNA Polymerase has no proofreading or hot start features. It is optimally active at +75°C and pH 9. The 5′ → 3′ DNA polymerase lacks 3′ → 5′ exonuclease activity. The thermostable enzyme is a single polypeptide chain with a molecular weight of approximately 95 kD.
Lack of restriction endonuclease:
The enzyme originally isolated from T. aquaticus BM lacks Taq I restriction endonuclease activity.
The PCR Master contains all the reagents required to perform a standard PCR. All that must be added is the template DNA, the primers, and the required amount of water. The PCR Master contains a fixed MgCl2 concentration of 1.5 mM. However, higher concentrations may be achieved by adding additional MgCl2. PCR products generated by PCR Master are 3′-A single-overhang products. Therefore T/A cloning can be applied.
The PCR Master is stable at +2 to +8 °C, allowing immediate PCR without the time-consuming thawing of reagents.

Application

The PCR Master can be used instead of the single reagent Taq DNA Polmerase, or instead of the PCR Core Kit for nearly all simple, routine polymerase chain reaction applications. The only limitation is that the sample volume must not exceed half the total reaction volume. The PCR master mix is used for:
  • Routine PCR
  • RT-PCR
  • Other primer-extension reactions, such as sequencing and labeling

Features and Benefits

  • Reliable reproducible results:
High lot-to-lot consistency.
  • Convenience:
Adding template and primer requires only two pipetting steps.
  • Prevent PCR carryover:
dUTP incorporation combination with Uracil-DNA Glycosylase prevents PCR cross-contamination.
  • Ready for immediate use:
Master mix can be stored at +2 to +8°C.

Packaging

1 kit containing 2 components.

Quality

Each lot is PCR tested using λDNA. Each lot is also tested for the absence of exo- and endonucleases, and nicking activities according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

Kit Components Only

Product No.
Description

  • PCR Master Mix (10 vials) 2x concentrated

  • Water, PCR Grade (5 vials)

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

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