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11666681001

Roche

Buffers in a Box, Premixed SSC Buffer, 20x

pH 7.0, pkg of 4 L, solution

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Synonym(s):
SSC buffer

form

solution

packaging

pkg of 4 L

manufacturer/tradename

Roche

pH

7.0

shipped in

ambient

storage temp.

20-25°C

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This Item
11666690001S09028410-OP
vibrant-m

S0902

SSC Buffer

vibrant-m

8410-OP

SSPE Buffer

pH

7.0

pH

8.4

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

pH

-

form

solution

form

solution

form

powder blend

form

liquid

packaging

pkg of 4 L

packaging

pkg of 4 L

packaging

-

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

ambient

General description

20x solution

Application

Buffers in a Box, Premixed SSC Buffer, 20x has been used:
  • as a wash buffer in in situ hybridization to determine the expression of miRNAs in paraffin-embedded tissue specimens
  • as a wash buffer in single-molecule fluorescent in situ hybridization (smFISH) protocol using U2-OS cells and primary neuronal cultures
  • as a component of rolling-circle amplification (RCA) hybridization buffer for use in fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissue samples

Features and Benefits

  • Stable 20x solution of saline sodium citrate (SSC) purified by filtration using a 0.2 μm pore size membrane
  • resistant to enzymatic and nonenzymatic degradation
  • free from nuclease or protease activity
  • can store at room temperature

Quality

Absence of contaminants: Free of DNase and RNase according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash


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Malgorzata J Latallo et al.
Methods (San Diego, Calif.), 162-163, 12-22 (2019-03-25)
The central dogma of molecular biology reaches a crescendo at its final step: the translation of an mRNA into its corresponding protein product. This process is highly regulated both spatially and temporally, requiring techniques to interrogate the subcellular translational status

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