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ROAHAHA

Roche

Anti-HA High Affinity

from rat IgG1

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Synonym(s):
antibody

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F10, monoclonal

form

lyophilized

packaging

pkg of 50 μg (11867423001)
pkg of 500 μg (11867431001)

manufacturer/tradename

Roche

isotype

IgG1

epitope sequence

YPYDVPDYA

storage temp.

2-8°C

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ROAHAHA

Anti-HA High Affinity

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biological source

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biological source

rabbit

biological source

mouse

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conjugate

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isotype

IgG1

isotype

IgG1

isotype

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IgG1

manufacturer/tradename

Roche

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General description

Anti-HA High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10). Anti-HA High Affinity recognizes the HA peptide sequence (YPYDVPDYA), derived from the influenza hemagglutinin protein. The antibody recognizes its antigenic determinant even when the HA peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

Specificity

Anti-HA, High Affinity recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Application

Use Anti-HA High Affinity for the detection of native influenza hemagglutinin protein and recombinant proteins that contain the HA epitope using:
  • Dot blots
  • ELISA
  • Immunocytochemistry
  • Immunoprecipitation
  • Western blots
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Quality

Function tested in western blot.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • ELISA: for detection 100 ng/ml; for coating 1 to 5 μg/ml
  • Immunoprecipitation: 0.5 to 5 μg/ml
  • Western blot: 50 to 200 ng/ml

Reconstitution

Add 0.5 ml (11 867 423 001; 50 μg) or 2.5 ml (11 867 431 001; 500 μg) double-distilled water to a final concentration of 100 μg/ml or 200 μg/ml respectively.
Rehydrate for 10 min prior to use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

pictograms

Exclamation markExclamation mark

signalword

Warning-Warning

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

does not flash

flash_point_c

does not flash


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