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2,3,5-Triphenyl-tetrazolium chloride solution

suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

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Synonym(s):
TTC solution, TTC supplement, TTC solution, TTC supplement
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
Beilstein/REAXYS Number:
3923356
MDL number:
PubChem Substance ID:
NACRES:
NA.85

Quality Level

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

mol wt

334.8 g/mol

application(s)

agriculture
environmental
food and beverages

microbiology

storage temp.

2-8°C

suitability

bacteria

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

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54232T9656T7791
vibrant-m

54232

Lactose TTC Agar with Tergitol-7

sterility

sterile (Filtered and Aseptic Handled)

sterility

non-sterile

sterility

-

sterility

-

form

liquid

form

powder

form

liquid

form

liquid

suitability

bacteria

suitability

selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms

suitability

-

suitability

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

shelf life

limited shelf life, expiry date on the label

shelf life

limited shelf life, expiry date on the label

shelf life

-

shelf life

-

General description

2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.

Application

2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media

Components

(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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