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68724

Supelco

Fast Green FCF

analytical standard

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Synonym(s):
Food green 3
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein/REAXYS Number:
5718212
EC Number:
MDL number:
PubChem Substance ID:
E Number:
E143
NACRES:
NA.24

grade

analytical standard

Quality Level

assay

≥98.0% (HPLC)

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

290 °C (dec.) (lit.)

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

InChI key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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This Item
F7252F725880717
vibrant-m

68724

Fast Green FCF

vibrant-m

F7252

Fast Green FCF

vibrant-m

F7258

Fast Green FCF

vibrant-m

80717

Brilliant Blue FCF

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

application(s)

cleaning products
cosmetics
food and beverages
personal care

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
food and beverages
personal care

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

-

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

grade

analytical standard

grade

-

grade

certified by the Biological Stain Commission

grade

analytical standard

assay

≥98.0% (HPLC)

assay

-

assay

-

assay

≥97.0% (HPLC)

General description

Fast Green FCF is a synthetic organic dye, which belongs to the triphenylmethane group. It can be found in a wide range of applications in food and beverage industry. It can also be used in scientific research for the staining of proteins and identification of extracellular recording or injection sites in electrophysiological experiments.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation markHealth hazard

signalword

Warning

Hazard Classifications

Muta. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

352.4 °F - closed cup

flash_point_c

178 °C - closed cup


Certificates of Analysis (COA)

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Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons
Hooft V.AJ
Neuroscience Letters, 318, 163-165 (2002)
Nonlinear responses and optical limiting behavior of fast green FCF dye under a low power CW He?Ne laser irradiation
Jamshidi-Ghaleh K, et al.
Optics Communications, 271, 551-554 (2007)
Sumiko Tsuji et al.
Journal of chromatography. A, 1101(1-2), 214-221 (2005-10-26)
The existence of five of the six expected isomers in commercial Fast Green FCF (G3: Food Green No. 3, FD&C Green No. 3, CAS No. 2353-45-9, C.I. No. 42053), the main product of which is m,m-G3 and the sub-products of
D Wittekind et al.
Analytical and quantitative cytology, 4(4), 286-294 (1982-12-01)
The morphologic and spectroscopic characteristics of a new and reproducible modification of the Papanicolaou stain are briefly described. The main features of this modification are (1) replacement of the natural dye hematoxylin by the synthetic and chemically well defined dye
A V Grigor'eva et al.
Tsitologiia, 27(2), 191-195 (1985-02-01)
A cytophotometrical study was made of Fast Green FCF binding by nuclear non-histone proteins of the rat superior ganglion neurons at pH 2.6 (1 day, 1 week, 1 and 5 months, and 2.5 years after birth, a. b.). An increased

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