MilliporeSigma
All Photos(2)

Documents

69198

Millipore

Fraser Broth, Base

suitable for microbiology, NutriSelect® Plus

Sign Into View Organizational & Contract Pricing

Synonym(s):
Listeria Selective Enrichement Broth, Base (acc. Fraser)
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

casein enzymic hydrolysate, 5.0 g/L
disodium phosphate dihydrate, 12.0 g/L
esculin, 1.0 g/L
lithium chloride, 3.0 g/L
meat extract, 5.0 g/L
peptic digest of animal tissue, 5.0 g/L
potassium dihydrogen phosphate, 1.35 g/L
sodium chloride, 20.0 g/L
yeast extract, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
pathogen testing
sample preparation

microbiology

suitability

selective and differential for Listeria spp.

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
598597018469527
Fraser Broth, Base suitable for microbiology, NutriSelect® Plus

69198

Fraser Broth, Base

Antibiotic Broth suitable for microbiology, NutriSelect® Plus

70184

Antibiotic Broth

suitability

selective and differential for Listeria spp.

suitability

selective and differential for Listeria spp.

suitability

bacteria, nonselective for Escherichia coli, nonselective for Klebsiella spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for coliforms

suitability

Staphylococcus spp.

form

powder

form

powder

form

powder

form

powder

composition

casein enzymic hydrolysate, 5.0 g/L , esculin, 1.0 g/L , meat extract, 5.0 g/L , potassium dihydrogen phosphate, 1.35 g/L , yeast extract, 5.0 g/L , disodium phosphate dihydrate, 12.0 g/L , peptic digest of animal tissue, 5.0 g/L , lithium chloride, 3.0 g/L , sodium chloride, 20.0 g/L

composition

ammonium ferric citrate, 0.5 g/L , esculin, 0.8 g/L , lithium chloride, 10.0 g/L , D-mannitol, 5.0 g/L , peptone (vegetable), 23.0 g/L , phenol red, 0.08 g/L , polysorbate 80, 2.0 g/L , soya lecithin, 1.0 g/L , yeast extract, 5.0 g/L

composition

dextrose, 1.0 g/L , dipotassium hydrogen phosphate, 3.68 g/L , meat extract, 1.5 g/L , peptone (from meat), 5.0 g/L , potassium dihydrogen phosphate, 1.32 g/L , sodium chloride, 3.5 g/L , yeast extract, 1.5 g/L

composition

beef extract, 5.0 g/L , casein enzymic hydrolysate, 10.0 g/L , glycine, 1.2 g/L , lithium chloride, 5.0 g/L , mannitol, 20.0 g/L , sodium chloride, 5.0 g/L , sodium pyruvate, 3.0 g/L , yeast extract, 5.0 KBE/mL

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

application(s)

agriculture
environmental
food and beverages
pathogen testing
sample preparation, microbiology

application(s)

agriculture
environmental
food and beverages
pathogen testing
sample preparation
veterinary, microbiology

application(s)

clinical testing
food and beverages
life science and biopharma
pharmaceutical, microbiology

application(s)

environmental
food and beverages
sample preparation, microbiology

Application

Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds

Preparation Note

Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired.
Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service