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03978

Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

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Synonym(s):
Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein/REAXYS Number:
3641570
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

Inchi Key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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1 of 4

This Item
180831911201363
vibrant-m

03978

Methylene Blue solution

vibrant-m

319112

Methylene Blue solution

vibrant-m

01363

Neisser′s Methylene Blue Solution

form

liquid

form

solution

form

liquid

form

liquid

shelf life

~3 yr

shelf life

-

shelf life

-

shelf life

-

concentration

1.5% (1.5 g/ 100 mL)

concentration

1.4 % (w/v) in 95% ethanol

concentration

0.05 wt. % in H2O

concentration

-

density

1.0 g/mL at 20 °C

density

-

density

0.98 g/mL at 25 °C

density

-

εmax

9.4 at 656-666 nm in water

εmax

-

εmax

-

εmax

-

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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