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137276

SAFC

BIS-TRIS

EMPROVE® EVOLVE

Synonym(s):
BIS-TRIS, 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
Empirical Formula (Hill Notation):
C8H19NO5
CAS Number:
Molecular Weight:
209.24
Beilstein:
2205275
MDL number:

biological source

synthetic

Quality Level

product line

EMPROVE® EVOLVE

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

water: 0.1 M, clear, colorless

suitability

suitable for manufacturing use

storage temp.

2-30°C

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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This Item
RES1161B-A7B9754391335
BIS-TRIS EMPROVE® EVOLVE

SAFC

137276

BIS-TRIS

BIS-TRIS

SAFC

RES1161B-A7

BIS-TRIS

BIS-TRIS ≥98.0% (titration)

Sigma-Aldrich

B9754

BIS-TRIS

BIS-Tris, ULTROL® Grade A zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature.

Millipore

391335

BIS-Tris, ULTROL® Grade

product line

EMPROVE® EVOLVE

product line

-

product line

-

product line

-

form

powder

form

powder

form

crystalline powder

form

solid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Endotoxin, microbial, and trace metals; tested

impurities

Endotoxin, microbial, and trace metals, tested

impurities

-

impurities

-

useful pH range

5.8-7.2

useful pH range

5.8-7.2

useful pH range

5.8-7.2

useful pH range

-

Application

Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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