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24324C

SAFC

EX-CELL® 302 Serum-Free Medium for CHO Cells

dry powder, suitable for cell culture

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MDL number:
MFCD01634638
NACRES:
NA.71

description

for research or for further manufacturing use

Quality Level

500

form

dry powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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24326C14324C24340C
SAFC

SAFC

24324C

EX-CELL® 302 Serum-Free Medium for CHO Cells

SAFC

SAFC

24326C

EX-CELL® 302 Serum-Free Medium for CHO Cells

SAFC

SAFC

14324C

EX-CELL® 302 Serum-Free Medium for CHO Cells

SAFC

SAFC

24340C

EX-CELL® 325 PF CHO Serum-Free Medium for CHO Cells

storage condition

protect from light

storage condition

protect from light

storage condition

protect from light

storage condition

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

description

for research or for further manufacturing use

description

Drug Master File available, for research or for further manufacturing use

description

for research or for further manufacturing use

description

for research or for further manufacturing use, without L-glutamine

Application

EX-CELL® 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or the GS System™, because it does not contain hypoxanthine, thymidine or L-glutamine. For use with the DHFR- selection system we recommend the addition of 4 mM L-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kDa. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Quantity

Formulated to contain 21.2 grams of powder per liter of medium.

Footnote

Ex-Cell 302 has also been used successfully with BHK-21 cells and chicken embryo-related (CER) cell lines.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Héla Kallel et al.
Journal of biotechnology, 95(3), 195-204 (2002-05-15)
We have carried out the adaptation of BHK-21 cells to two serum free (Ex Cell 520 and HyQ PF CHO) and three animal protein free media: Ex Cell 302, HyQ PF CHO MPS and Rencyte BHK. After a direct switch

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