MilliporeSigma
All Photos(1)

24326C

SAFC

EX-CELL® 302 Serum-Free Medium for CHO Cells

without L-glutamine, without phenol red, without sodium bicarbonate, dry powder, suitable for cell culture

All Photos(1)
MDL number:
MFCD01634638
NACRES:
NA.71

description

Drug Master File available
for research or for further manufacturing use

Quality Level

500

form

dry powder

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable (suspension)

pH

7.1-7.7

shipped in

ambient

storage temp.

2-8°C

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24326C

EX-CELL® 302 Serum-Free Medium for CHO Cells

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14326C

EX-CELL® 302 Serum-Free Medium for CHO Cells

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24360C

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

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24324C

EX-CELL® 302 Serum-Free Medium for CHO Cells

form

dry powder

form

liquid

form

dry powder

form

dry powder

storage condition

protect from light

storage condition

protect from light

storage condition

-

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

Application

EX-CELL® 302 is a serum-free medium, which has been specifically developed for the long-term growth of transformed Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or protein products. EX-CELL 302 is an appropriate medium for use with the DHFR- or Glutamine Synthetase, or GS System™ because it does not contain hypoxanthine, thymidine or L-glutamine. If EX-CELL 302 is to be used with CHO cells transformed using the GS selection system the media needs to be supplemented with GS Supplement 50X for the additional amino acids and nucelosides. For use with the DHFR- selection system we recommend the addition of 4 mM L-Glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, EX-CELL 302 was developed using only recombinant human proteins that have molecular weights less than 10 kD. The total protein concentration found in EX-CELL 302 is less than 1 mg/L. Pluronic™ F68 has been added at a final concentration of 0.1% to EX-CELL 302 to protect against shear damage in sparged bioreactor systems.

Caution

Do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.

Quantity

Formulated to contain 21.2 grams of powder per liter of medium

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
p3xFLAG is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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