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24581C

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EX-CELL® MDCK Serum-Free Medium for MDCK Cells

Animal-protein free, with L-glutamine, without sodium bicarbonate, dry powder, suitable for cell culture

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MDL number:
MFCD01635225
NACRES:
NA.71

Quality Level

500

description

for research or for further manufacturing use

form

dry powder

availability

not available in Japan

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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14581C

EX-CELL® MDCK Serum-Free Medium for MDCK Cells

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

-

description

for research or for further manufacturing use

description

for research or for further manufacturing use

description

for research or for further manufacturing use

description

for research or for further manufacturing use

availability

not available in Japan

availability

-

availability

-

availability

-

Application

EX-CELL MDCK is an animal-protein free, serum-free medium developed for the long-term growth of Madin Darby Canine Kidney (MDCK) and related cells. The cells, in an attachment culture, can be subcultured directly into EX-CELL MDCK from serum-supplemented media without adaptation. Cell densities and doubling times achieved under serum-free conditions are comparable to those achieved in a serum-supplemented culture.

Quantity

Formulated to contain 21.6 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Jonathan Liu et al.
Vaccine, 27(46), 6460-6463 (2009-06-30)
To develop a cell-based live attenuated influenza vaccine (LAIV) manufacturing process, several different cell lines were evaluated by comparing the titer of viruses after infection with LAIV strains. While several cell lines have been reported to support influenza virus replication
Yvonne Genzel et al.
Applied microbiology and biotechnology, 88(2), 461-475 (2010-07-10)
Over the last decade, adherent MDCK (Madin Darby canine kidney) and Vero cells have attracted considerable attention for production of cell culture-derived influenza vaccines. While numerous publications deal with the design and the optimization of corresponding upstream processes, one-to-one comparisons
Colin A Russell et al.
Science (New York, N.Y.), 336(6088), 1541-1547 (2012-06-23)
Avian A/H5N1 influenza viruses pose a pandemic threat. As few as five amino acid substitutions, or four with reassortment, might be sufficient for mammal-to-mammal transmission through respiratory droplets. From surveillance data, we found that two of these substitutions are common
Manuel J Deutsch et al.
Journal of virology, 84(5), 2533-2546 (2009-12-25)
The Epstein-Barr virus efficiently infects human B cells. The EBV genome is maintained extrachromosomally and replicates synchronously with the host's chromosomes. The latent origin of replication (oriP) guarantees plasmid stability by mediating two basic functions: replication and segregation of the

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