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379M-1

Sigma-Aldrich

Carbonic Anhydrase IX (CA IX) (MRQ-54) Mouse Monoclonal Antibody

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NACRES:
NA.41

biological source

mouse

Quality Level

100
500

conjugate

unconjugated

antibody form

culture supernatant

antibody product type

primary antibodies

clone

MRQ-54, monoclonal

description

For In Vitro Diagnostic Use in Select Regions (See Chart)

form

buffered aqueous solution

species reactivity

human

packaging

vial of 0.1 mL concentrate (379M-14)
vial of 0.5 mL concentrate (379M-15)
bottle of 1.0 mL predilute (379M-17)
vial of 1.0 mL concentrate (379M-16)
bottle of 7.0 mL predilute (379M-18)

manufacturer/tradename

Cell Marque

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200

isotype

IgG2b

control

clear cell renal cell carcinoma, gallbladder epithelium

shipped in

wet ice

storage temp.

2-8°C

visualization

membranous

Gene Information

human ... CA9(768)

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This Item
379R-1SAB5701575325M-1
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

clone

MRQ-54, monoclonal

clone

EP161, monoclonal

clone

monoclonal

clone

OC125, monoclonal

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

human

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

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General description

CA IX are a family of zinc containing metalloproteins that catalyze the reversible hydration of CO2. Among these, CA IX is anchored to the cell membrane and is expressed in the human gastrointestinal tract, chiefly in the stomach and gall bladder. It is interesting to note that CA IX is overexpressed in epithelial malignancies of the uterus, cervix, lung, breast, and kidney; none of the associated normal tissues express this isozyme. CA IX is said to maintain the extracellular acidic pH, thus promoting cell growth in these tumors5. Preliminary data suggest consistent immunoreactivity for anti-CA IX in clear cell renal cell carcinoma (RCC); it has sensitivity of 85% to 100% for clear cell RCC and this imparts diagnostic utility. Anti-CA IX may also play a role in distinguishing clear cell RCC from chromophobe RCC. Anti-CA IX, together with antibodies against Pax 2, Ksp-cadherin, and CD117, forms a robust panel that can be used to make this distinction. Strong diffuseto-multifocal immunostaining for anti-CA IX is observed in the large majority of urothelial carcinomas as opposed to the extremely weak and focal immunoreactivity seen in collecting duct carcinoma (CDC). Anti-CA IX can thus aid in distinguishing between urothelial carcinoma and CDC6.

Quality


IVD

NA

NA

NA

Linkage

Carbonic Anhydrase IX (CA IX) Positive Control Slides, Product No. 379S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Hikmat A Al-Ahmadie et al.
The American journal of surgical pathology, 32(3), 377-382 (2008-02-28)
Carbonic anhydrase IX (CAIX)-a protein maintaining intracellular and extracellular pH-reportedly also influences regulation of cell proliferation, oncogenesis, and tumor progression. Its expression is von Hippel-Lindau-hypoxia inducible factor pathway dependent. Immunohistochemical (IHC) studies show that CAIX is diffusely overexpressed in clear
Thambi Dorai et al.
Cancer investigation, 24(8), 754-779 (2006-12-13)
Renal cell carcinoma (RCC) has the highest rate of occurrence within the US when compared with other countries. Recent advances in the basic research and molecular diagnostics of this malignancy have revealed that RCC is not a single disease, but
S Ivanov et al.
The American journal of pathology, 158(3), 905-919 (2001-03-10)
An acidic extracellular pH is a fundamental property of the malignant phenotype. In von Hippel-Lindau (VHL)-defective tumors the cell surface transmembrane carbonic anhydrase (CA) CA9 and CA12 genes are overexpressed because of the absence of pVHL. We hypothesized that these
Mari Leppilampi et al.
World journal of gastroenterology, 9(7), 1398-1403 (2003-07-11)
To systematically study the expression of carbonic anhydrase (CA) isozymes IX and XII in gastric tumors. We analyzed a representative series of specimens from non-neoplastic gastric mucosa and from various dysplastic and neoplastic gastric lesions for the expression of CA
A J Kivelä et al.
Histochemistry and cell biology, 114(3), 197-204 (2000-11-18)
Carbonic anhydrase (CA) IX and XII are transmembrane isoenzymes which are expressed in several epithelia and overexpressed in some carcinomas. They have recently been linked to von Hippel-Lindau gene-mediated carcinogenesis in that both isoenzymes are downregulated by the product of

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