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50950

Sigma-Aldrich

Guanidine hydrochloride

≥98%

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Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.55

Assay

≥98%

form

powder or crystals

storage condition

(Tightly closed. Dry. )

color

colorless to white

pH

(25 °C, 4.6 - 6/573 g/L)

mp

180-185 °C (lit.)

solubility

H2O: 6 M, clear (100 Hazen)

density

1.3 g/cm3 (lit.)

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

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1 of 4

This Item
RDD00150933G9284
Guanidine hydrochloride ≥98%

Sigma-Aldrich

50950

Guanidine hydrochloride

-
Guanidine hydrochloride anhydrous, free-flowing, Redi-Dri™, ≥99%

Sigma-Aldrich

RDD001

Guanidine hydrochloride

-
Guanidine hydrochloride BioUltra, for molecular biology, ≥99.5% (AT)

Sigma-Aldrich

50933

Guanidine hydrochloride

Premium Grade
form

powder or crystals

form

-

form

powder or crystals

form

liquid

storage condition

(Tightly closed. Dry. )

storage condition

-

storage condition

-

storage condition

-

color

colorless to white

color

-

color

colorless to white,

color

-

pH

(25 °C, 4.6 - 6/573 g/L)

pH

(25 °C, 4.6 - 6/573 g/L)

pH

4.5-6.0 (25 °C, 6 M in H2O)

pH

-

mp

180-185 °C (lit.)

mp

180-185 °C (lit.)

mp

180-185 °C (lit.)

mp

-

General description

Guanidine hydrochloride, or aminoformamidine hydrochloride, is a versatile chemical compound widely used in cell biology and biochemical research due to its potent chaotropic properties. Its ability to disrupt protein and nucleic acid structures makes it valuable for purifying proteins and nucleic acids, particularly mRNA. Guanidine hydrochloride unfolds proteins, facilitating their purification and refolding, and it is effective in solubilizing denatured insoluble proteins at higher concentrations.

At lower concentrations, guanidine hydrochloride has the intriguing ability to promote the refolding of denatured proteins, aiding in protein renaturation studies. In RNA extraction, it acts as a strong denaturant, disrupting cell structures and ensuring the integrity of extracted RNA by inactivating RNA enzymes. Overall, guanidine hydrochloride′s denaturing and renaturing properties make it an essential reagent for various cell biology applications, including protein purification, nucleic acid isolation, and protein refolding studies.

Application

Guanidine hydrochloride has been used:
  • for lysing homogenized brain tissue
  • in the preparation of incubation buffer for Ni-sepharose protein binding, purification, propionylation, and on-bead digestion to minimize nonspecific binding to the affinity resin
  • in the preparation of solutions/extraction buffer to extract soluble protein from the human tissue

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride, an efficient chaotropic reagent, is involved in RNA isolation, globular protein denaturation, and protein refolding studies. This denaturant is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.

Features and Benefits

Highly versatile surfactant for your cell biology and biochemical research

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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