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94506

Sigma-Aldrich

Tetramethylrhodamine-5-maleimide

suitable for fluorescence, ≥85% (HPLC)

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Empirical Formula (Hill Notation):
C28H23N3O5
CAS Number:
Molecular Weight:
481.50
MDL number:
NACRES:
NA.32

Quality Level

assay

≥85% (HPLC)

form

powder

solubility

DMSO: soluble
methanol: soluble

fluorescence

λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

InChI

1S/C28H25N3O5/c1-29(2)16-5-9-20-23(14-16)36-24-15-17(30(3)4)6-10-21(24)27(20)19-8-7-18(13-22(19)28(34)35)31-25(32)11-12-26(31)33/h5-9,11-15H,10H2,1-4H3,(H,34,35)

InChI key

KGFLZYXDJDOIEE-UHFFFAOYSA-N

Related Categories

Application

Tetramethylrhodamine-5-maleimide has been used:
  • to concentrate the eluate and in labeling to create IRE1LDS112C
  • to derivatize green fluorescent protein (GFP) on Cys48 for experiments with the extrinsic fluorescent probe
  • to incubate the single solvent exposed cys mutant to perform protein labeling

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Labeling reagent for protein thiols for cytofluorescence

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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