A9539

Agarose

BioReagent, for molecular biology, low EEO

• Synonym(s): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE
• CAS Number: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• PubChem Substance ID: 24891469
• NACRES: NA.25

Properties

• biological source: algae (marine)
• Quality Level: 200
• grade: for molecular biology
• product line: BioReagent
• form: powder
• technique(s): electrophoresis: suitable
• impurities: ≤10% moisture content
• EEO: 0.09-0.13
• transition temp: gel point 36 °C ±1.5 °C (1.5% gel)
• gel strength: ≥1200 g/cm² (1% gel)
• anion traces: sulfate (SO₄^2-): ≤0.15%
• foreign activity: DNase, RNase, none detected
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

Application

Routine use agarose is ideal for everyday analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). Has low ethidium bromide and SYBR Green background staining.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves