CMC0014

BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

• Synonym(s): Competent E. coli cells
• UNSPSC Code: 41106202
• NACRES: NA.85

Properties

• product name: BL21(DE3) Chemically Competent Cells, for protein expression
• biological source: Escherichia coli
• grade: for molecular biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: chemically competent; transformation efficiency: ≥1 x 10⁷ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Application

The E. coli strain BL21 (DE3) has been used:

• to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
• BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
• to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Features and Benefits

• a transformation efficiency of >1 × 10⁷ cfu/μg.
• economical prices
• offering superior value for everyday protein expression work.

Components

• BL21(DE3) chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Safety Information

• Storage Class: 10 - Combustible liquids