D8906

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average M_w >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

• Synonym(s): 6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Manninotriose
• CAS Number: 9011-18-1
• MDL number: MFCD00081551
• NACRES: NA.31

Properties

• biological source: synthetic
• Quality Level: 300
• grade: for molecular biology
• form: powder
• mol wt: average M_w >500,000 (dextran starting material)
• contains: 0.5-2% phosphate buffer
• composition: Sulfur Content, 16.0-19.0%
• color: off-white
• useful pH range: 6.0-8 (10 g/L)
• solubility: H₂O: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.

Application

Dextran sulfate has been used

• as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis
• as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis

Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Biochem/physiol Actions

Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.

Features and Benefits

• Suitable for Nucleic acid hybridization & Molecular Biology
• Dextran Sulfate with an average molecular weight of >500,000
• Sulfur Content of 16.0-19.0 %
• Versatile and adaptable for wide variety of laboratory and research applications

Suitability

Tested for suitability in nucleic acid hybridizations

Other Notes

This product is intended for research purposes only, and it is not meant for human consumption.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves