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E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid

anhydrous, crystalline, BioReagent, suitable for cell culture

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Synonym(s):
(Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid
Linear Formula:
(HO2CCH2)2NCH2CH2N(CH2CO2H)2
CAS Number:
Molecular Weight:
292.24
Beilstein:
1716295
EC Number:
MDL number:
eCl@ss:
39030907
PubChem Substance ID:
NACRES:
NA.25

grade

anhydrous

product line

BioReagent

Assay

≥98.5%

form

crystalline

reaction suitability

reagent type: chelator

technique(s)

cell culture | mammalian: suitable

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

3  M NaOH: 100 mg/mL

density

1.46 g/cm3 at 20 °C

cation traces

C: 40.7-41.5%
N: 9.3-9.8%

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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This Item
EDSRDD017798681
Ethylenediaminetetraacetic acid anhydrous, crystalline, BioReagent, suitable for cell culture

Sigma-Aldrich

E6758

Ethylenediaminetetraacetic acid

Essential+ Grade
Ethylenediaminetetraacetic acid BioUltra, anhydrous, ≥99% (titration)

Sigma-Aldrich

EDS

Ethylenediaminetetraacetic acid

Premium Grade
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri™, ACS reagent, 99.4-100.6%

Sigma-Aldrich

RDD017

Ethylenediaminetetraacetic acid

Essential Grade
product line

BioReagent

product line

BioUltra

product line

Redi-Dri

product line

Redi-Dri

assay

≥98.5%

assay

≥99% (titration)

assay

99.4-100.6%

assay

≥98%

form

crystalline

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

2.5 (23 °C, 10 g/L)

pH

-

Application

Ethylenediaminetetraacetic acid has been used:
  • as a component of peptide dilution buffer for intracellular BH3 (Bcl-2 homology (BH) domain) profiling and validation
  • as a medium component in the hepatic stellate cell (HSCs) culture
  • as a component of homogenization buffer for protein extraction from eye tissues
  • for stopping benzonase reaction in lysis buffer

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood samples. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline imparts serves as root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium Disodium EDTA administration is effective in slowing down the progression of chronic kidney disease.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Protein Expressions of the Small GTPase Rho Proteins in Pterygial Tissue and Leukocytes of Patients with Pterygium
Saracaloglu A, et al.
Proceedings, 2(25), 1571-1571 (2018)
Analysis of Direct Interaction between Viral DNA-binding Proteins by Protein Pull-down Co-immunoprecipitation Assay
Lechuga A, et al.
Bio-protocol, 8(1), 1571-1571 (2018)
The blockade of lipophagy pathway is necessary for docosahexaenoic acid to regulate lipid droplet turnover in hepatic stellate cells
Qiu S, et al.
Biomedicine and Pharmacotherapy, 109, 1841-1850 (2019)
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation
Dai J and Luftig MA
Journal of Immunology, ji1701473-ji1701473 (2018)
Calcification in coronary artery disease can be reversed by EDTA-tetracycline long-term chemotherapy
Maniscalco BS and Taylor KA
Pathophysiology : The Official Journal of the International Society for Pathophysiology / ISP, 11(2), 95-101 (2004)

Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

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