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F4523

Sigma-Aldrich

SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets

tablet, To prepare 10 mL

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Synonym(s):
4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets
MDL number:
NACRES:
NA.83

form

tablet

storage temp.

−20°C

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This Item
F4648D4293N2770
storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

SIGMAFAST Fast Red TR/Naphthol AS-MX tablets has been used in fluorescent in situ hybridization for the analysis of gene expression in neurons and synapses.
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.

Reconstitution

Each tablet set dissolved in 10 mL of deionized water yields a ready-to-use buffered solution.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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K Buchmann
Folia parasitologica, 45(4), 312-318 (1998-12-30)
Histochemical studies of enzyme activities and structural elements in Gyrodactylus derjavini Mikailov, 1975 parasitizing fins of Oncorhynchus mykiss Walbaum were conducted. Marked activities of non-specific esterase, acid phosphatase, alkaline phosphatase and amino-peptidase were found in the intestinal caeca of the
G B Baretton et al.
British journal of cancer, 80(3-4), 546-555 (1999-07-17)
The molecular mechanisms leading to androgen-independent growth in prostate cancer (PC) are poorly understood. Androgen deprivation therapy (ADT) results physiologically in a decrease in proliferation and an increase in programmed cell death (PCD)/apoptosis. The aim of our study was to
Calcium imaging of basal forebrain activity during innate and learned behaviors
Harrison TC, et al.
Frontiers in Neural Circuits, 10, 36-36 (2016)
The
In Situ Hybridization Methods (2015)
Andrea M Mess et al.
Reproductive biology and endocrinology : RB&E, 10, 102-102 (2012-12-04)
Since Xenarthra are serious candidates for being basal to Eutheria, their characteristics, e.g. the placental system, influence perceptions of evolution. However, in the subgroup containing the anteaters, data are very limited. The present study aims to elucidate the nature of

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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