MilliporeSigma
All Photos(1)

Documents

S6402

Sigma-Aldrich

Streptavidin−Cy3™ from Streptomyces avidinii

buffered aqueous solution

All Photos(1)
MDL number:
MFCD00164141

conjugate

CY3 conjugate

Quality Level

200

form

buffered aqueous solution

extent of labeling

3-9 mol fluorochrome per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
S9415S5512S3762
Sigma-Aldrich

Sigma-Aldrich

S6402

Streptavidin−Cy3™ from Streptomyces avidinii

Sigma-Aldrich

Sigma-Aldrich

S9415

Streptavidin−Maleimide from Streptomyces avidinii

Streptavidin−Peroxidase from Streptomyces avidinii lyophilized powder

Sigma-Aldrich

S5512

Streptavidin−Peroxidase from Streptomyces avidinii

Streptavidin−FITC from Streptomyces avidinii essentially salt-free, lyophilized powder, ≥5 units/mg protein

Sigma-Aldrich

S3762

Streptavidin−FITC from Streptomyces avidinii

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

-

technique(s)

direct ELISA: 1:50,000

technique(s)

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

conjugate

CY3 conjugate

conjugate

-

conjugate

peroxidase conjugate

conjugate

FITC conjugate

extent of labeling

3-9 mol fluorochrome per mol protein

extent of labeling

4-10 mol maleimide per mol Streptavidin

extent of labeling

-

extent of labeling

3-9 mol FITC per mol streptavidin

General description

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

Application

Streptavidin−Cy3™ from Streptomyces avidinii has been used:
  • in double immunofluorescence staining to detect biotinylated lectin
  • for insulin and C-peptide staining in immunofluorescence analysis
  • in double immunofluorescence to verify the presence of cells filled with biocytin
  • in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
  • with the traditional rhodamine filter arrangements due to the spectral overlap

Features and Benefits

Spectral Characteristics of Cy3 Dye:
  • Absorbance Max 552 nm
  • Emission Max 565 nm
  • Molar Extinction Coefficient 1.3 x 10-5 M-1cm-1

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Legal Information

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Hiroyuki Nobusue et al.
Cell and tissue research, 332(3), 435-446 (2008-04-04)
We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
Agnieszka E Gorska et al.
The American journal of pathology, 163(4), 1539-1549 (2003-09-26)
We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
Niklas Schwarz et al.
eLife, 8 (2019-09-10)
Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel
Vera S Donnenberg et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 93(4), 448-457 (2018-03-03)
Flow cytometric cell surface proteomics provides a new and powerful tool to determine changes accompanying neoplastic transformation and invasion, providing clues to essential interactions with the microenvironment as well as leads for potential therapeutic targets. One of the most important
View All Related Papers

Articles

Fluorescent in situ Hybridization (FISH)

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service