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A0410

Sigma-Aldrich

Aminoallyl-dUTP sodium salt

≥85%, lyophilized powder

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Synonym(s):
5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
Molecular Weight:
523.22 (free acid basis)
NACRES:
NA.51

Quality Level

assay

≥85%

form

lyophilized powder

shipped in

dry ice

storage temp.

−70°C

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This Item
A5660A677911051440001
vibrant-m

A0410

Aminoallyl-dUTP sodium salt

vibrant-m

A5660

Aminoallyl-UTP sodium salt

vibrant-m

11051440001

dATP

assay

≥85%

assay

≥75%

assay

≥97%

assay

98%

form

lyophilized powder

form

lyophilized powder

form

powder

form

solution

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−70°C

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

Application

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
  • to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
  • for the generation of chromatin immunoprecipitation (ChIP) probes
  • in the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Kevin K Fuller et al.
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Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening
Eriksson E M, et al.
Plant Physiology, 136(4), 4184-4197 (2004)
Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
Testa A, et al.
The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
W Gregory Cox et al.
BioTechniques, 36(1), 114-122 (2004-01-27)
Fluorescent nucleic acid hybridization probes traditionally have been generated by enzymatic incorporation of dye-labeled nucleotides, even though incorporation efficiency is low and variable from dye to dye. Alternatively, 5-(3-aminoallyl)-2'-deoxyuridine 5'-triphosphate (aa-dUTP) is enzymatically incorporated to generate amine-modified DNA, which is
Probe production for in situ hybridization by PCR and subsequent covalent labeling with fluorescent dyes.
Dirsch O, Ji Y, Bohr J, et al.
Appl. Immunohistochem., 15, 332-337 (2007)

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