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A0919

Sigma-Aldrich

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing preservative.)

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MDL number:
NACRES:
NA.46

conjugate

agarose conjugate

Quality Level

antibody form

purified immunoglobulin

form

(Suspension in 0.5 M NaCl containing preservative.)

extent of labeling

1 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

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A1047A3438G7777
vibrant-m

A0919

Mouse IgG−Agarose

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

form

(Suspension in 0.5 M NaCl containing preservative.)

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

aqueous glycerol suspension

technique(s)

immunoprecipitation (IP): suitable

technique(s)

direct ELISA: 1:7,000-1:21,000, western blot: suitable

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

extent of labeling

1 mg per mL

extent of labeling

-

extent of labeling

-

extent of labeling

-

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Application

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays,.
Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Veronica Cloud et al.
eLife, 8 (2019-07-28)
Atxn7, a subunit of SAGA chromatin remodeling complex, is subject to polyglutamine expansion at the amino terminus, causing spinocerebellar ataxia type 7 (SCA7), a progressive retinal and neurodegenerative disease. Within SAGA, the Atxn7 amino terminus anchors Non-stop, a deubiquitinase, to
Identification of in vivo phosphorylation sites and their functional significance in the sodium iodide symporter.
Vadysirisack, D. et. Al
The Journal of Biological Chemistry, 282, 3620-3628 (2007)
Robert C Alver et al.
Nucleic acids research, 42(13), 8389-8404 (2014-06-29)
Accurate replication of the genome requires the evolutionarily conserved minichromosome maintenance protein, Mcm10. Although the details of the precise role of Mcm10 in DNA replication are still debated, it interacts with the Mcm2-7 core helicase, the lagging strand polymerase, DNA
Chiang-Min Cheng et al.
Molecular and cellular biology, 31(5), 983-997 (2010-12-30)
Ras GTPases were long thought to function exclusively from the plasma membrane (PM). However, a current model suggests that Ras proteins can compartmentalize to regulate different functions, and an oncogenic H-Ras mutant that is restricted to the endomembrane can still
Timothy D Donaldson et al.
Molecular biology of the cell, 15(11), 4892-4903 (2004-08-28)
Cullin-dependent ubiquitin ligases regulate a variety of cellular and developmental processes by recruiting specific proteins for ubiquitin-mediated degradation. Cullin proteins form a scaffold for two functional modules: a catalytic module comprised of a small RING domain protein Roc1/Rbx1 and a

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