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A7732

Sigma-Aldrich

Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

clone EA-53, purified from hybridoma cell culture

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Synonym(s):
Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582, Anti-1110008F24Rik, Sarcomeric Alpha Actinin Antibody, Sarcomeric Alpha Actinin Antibody - Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

EA-53, monoclonal

form

buffered aqueous solution

species reactivity

cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using protease-digested sections of human tongue.
western blot: 2-4 μg/mL using rat skeletal muscle extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rabbit ... ACTN3(100009544)
rat ... Actn2(291245)

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This Item
A7811SAB4200602SAB4200689
species reactivity

cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken

species reactivity

mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard

species reactivity

human, carp, bovine, frog, sheep, rabbit, rat, snake, guinea pig

species reactivity

rat, chicken, bovine, mouse, guinea pig, rabbit, human, planaria(flatworm), fish, Xenopus

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rabbit ... ACTN3(100009544)
rat ... Actn2(291245)

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)

Gene Information

human ... ACTA1(58)

Gene Information

human ... ACTA2(59)

clone

EA-53, monoclonal

clone

EA-53, monoclonal

clone

5C5, monoclonal

clone

5C5, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

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General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-Actinin belongs to the spectrin protein superfamily.
α-Actinin dimerizes in an antiparallel fashion via interaction of the central rod domains. Four isoforms are found in human and mice. α-actinin-1 is ubiquitously expressed and located primarily in focal adhesions, whereas α-actinin-4 is present in certain membrane ruffles and seems to play a role in endocytosis and tumor cell motility.
ACTN1 (α-actinin-1) and ACTN4 (α-actinin-4) encodes non-muscle proteins, while ACTN2 (α-actinin-2) and ACTN3 (α-actinin-3) encodes muscle proteins.
Monoclonal Anti-α-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish α-actinin.

Specificity

Monoclonal Anti-α-Actinin (Sarcomeric) specifically recognizes α-skeletal muscle actinin and α-cardiac muscle actinin.

Immunogen

Purified rabbit skeletal α actinin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (2 papers)
Immunohistochemistry (1 paper)
Mouse Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody has been used for immunocytochemical (1:800) and immunohistochemical (1:100) applications.
Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody.

Biochem/physiol Actions

α-Actinin maintains the structure of cytoskeletal organization and regulates muscle contraction. α-Actinin functions as an actin crosslinker and promotes cell migration. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Distribution of alpha-actinin in single isolated smooth muscle cells
Fay F, et al.
The Journal of Cell Biology, 96(3), 783?795-783?795 (1983)
The integrin--actin connection, an eternal love affair.
Brakebusch Cord and Fassler R
The Embo Journal, 22(10), 2324-2333 (2003)
α-Actinin structure and regulation
Sjoblom B, et al.
Cellular and Molecular Life Sciences, 65(17), 2688-2688 (2008)
Zhaokang Cheng et al.
European heart journal, 32(17), 2179-2188 (2011-01-14)
The cascade of events leading to compromised mitochondrial integrity in response to stress is mediated by various combinatorial interactions of pro- and anti-apoptotic molecules. Nur77, an immediate early gene that encodes a nuclear orphan receptor, translocates from the nucleus to
Feifei Su et al.
JCI insight, 1(19), e90931-e90931 (2016-11-25)
Bcl-2-associated athanogene 3 (BAG3) is an evolutionarily conserved protein expressed at high levels in the heart and the vasculature and in many cancers. While altered BAG3 expression has been associated with cardiac dysfunction, its role in ischemia/reperfusion (I/R) is unknown.

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