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A8229

Sigma-Aldrich

Monoclonal Anti-A2B5 antibody produced in mouse

clone 105, purified immunoglobulin, lyophilized powder

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MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

105, monoclonal

form

lyophilized powder

species reactivity

human, rat, chicken, mouse

technique(s)

flow cytometry: 2.5 μg using using retinoic acid-differentiated NTera-2 human testicular embryonic carcinoma cell line (per 106 cells)
immunocytochemistry: 8-25 μg/mL using using NTera-2 human cell line

isotype

IgM

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
O7014SAB4200646P0493
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

105, monoclonal

clone

O1, monoclonal

clone

ALIX-1, monoclonal

clone

PRMT5-21, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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General description

A2B5 is a ganglioside that has recently been used as marker for stem cells.
Monoclonal Anti-A2B5, Clone 105, was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with embryonic chicken retinal cells.
Mouse Monoclonal Anti-A2B5 antibody binds to human, rat, chicken and mouse A2B5.

Immunogen

embryonic chicken retinal cells.

Application

Mouse Monoclonal Anti-A2B5 antibody can be used for flow cytometry (2.5μg) and immunocytochemistry (8-25μg/ml) assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Nagako Kawashima et al.
Journal of neurochemistry, 111(4), 1031-1041 (2009-09-22)
Sandhoff disease is a progressive neurodegenerative disorder caused by mutations in the HEXB gene which encodes the beta-subunit of N-acetyl-beta-hexosaminidase A and B, resulting in the accumulation of the ganglioside GM2. We isolated astrocytes from the neonatal brain of Sandhoff
Mikko T Huuskonen et al.
The Journal of experimental medicine, 219(1) (2021-12-01)
Subcortical white matter (WM) stroke accounts for 25% of all strokes and is the second leading cause of dementia. Despite such clinical importance, we still do not have an effective treatment for ischemic WM stroke, and the mechanisms of WM
C M Wen et al.
Journal of fish biology, 90(1), 201-221 (2016-10-13)
In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein
Cristina Martínez-Ramos et al.
Journal of tissue engineering and regenerative medicine, 13(3), 509-521 (2019-02-07)
Spinal cord injuries (SCIs) result in the loss of sensory and motor function with massive cell death and axon degeneration. We have previously shown that transplantation of spinal cord-derived ependymal progenitor cells (epSPC) significantly improves functional recovery after acute and
Tomoko Yamashita et al.
PloS one, 12(2), e0171947-e0171947 (2017-02-14)
Oligodendrocytes myelinate axons and form myelin sheaths in the central nervous system. The development of therapies for demyelinating diseases, including multiple sclerosis and leukodystrophies, is a challenge because the pathogenic mechanisms of disease remain poorly understood. Primate pluripotent stem cell-derived

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