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A8914

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)−Agarose antibody produced in goat

affinity isolated antibody, saline suspension

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

agarose conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

saline suspension

extent of labeling

~1 mg per mL

technique(s)

immunoelectrophoresis: suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

capacity

≥0.25 mg/mL, resin binding capacity (rabbit IgG)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A9294A3543A3316
technique(s)

immunoelectrophoresis: suitable

technique(s)

immunoelectrophoresis: suitable

technique(s)

immunoelectrophoresis: suitable

technique(s)

Ouchterlony double diffusion: suitable

biological source

goat

biological source

rabbit

biological source

goat

biological source

goat

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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General description

IgG antibodies are found in all body fluids and are capable of fighting bacterial and viral infection. It is the only type of antibody capable of crossing the placenta and immunising the foetus. Anti-rabbit IgG (whole molecule)-agarose antibody can be used in immunoprecipitation. IgGs are added to nuclear extracts of breast cancer cell lines then immunoprecipitation carried out. Anti-rabbit IgG (whole molecule)-agarose antibody can react specifically to all rabbit immunoglobulin.

Immunogen

Purified rabbit IgG.

Application

Anti-rabbit IgG (whole molecule)-agarose antibody can be used in immunoprecipitation and immunoelectrophoresis.
Protein depletion from mouse intestinal cytosol samples was accomplished by incubating the samples with a specific primary and the pulling down with agarose-linked goat anti-rabbit IgG.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Shahzad Siddiqi et al.
Journal of lipid research, 51(5), 1093-1100 (2009-12-08)
The rate-limiting step in the transit of dietary fat across the intestinal absorptive cell is its exit from the endoplasmic reticulum (ER) in a specialized ER-to-Golgi transport vesicle, the prechylomicron transport vesicle (PCTV). PCTV bud off from the ER membranes
The TACC proteins are coregulators of the Retinoid X Receptor b.
Ananthalaksmy K Vettaikkorumakankauv
Cancer Therapy, 6, 805-816 (2008)
Ronja Markworth et al.
Journal of cell science, 134(20) (2021-09-07)
Axonal survival and growth requires signalling from tropomyosin receptor kinases (Trks). To transmit their signals, receptor-ligand complexes are endocytosed and undergo retrograde trafficking to the soma, where downstream signalling occurs. Vesicles transporting neurotrophic receptors to the soma are reported to
Ronja Markworth et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 39(30), 5842-5860 (2019-05-28)
Neural circuit development involves the coordinated growth and guidance of axons. During this process, axons encounter many different cues, but how these cues are integrated and translated into growth is poorly understood. In this study, we report that receptor signaling

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