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B3111

Sigma-Aldrich

ANTI-FLAG® M2 antibody, Mouse monoclonal

Clone M2, purified from hybridoma cell culture in bioreactor

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Synonym(s):
Anti-ddddk, Anti-dykddddk, M2 clone ANTI-FLAG

biological source

mouse

antibody form

purified immunoglobulin (purified IgG1 subclass)

clone

M2, monoclonal

shelf life

4 yr

purified by

using Protein A

storage temp.

−20°C

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This Item
F1804A9469A8592
antibody form

purified immunoglobulin (purified IgG1 subclass)

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shelf life

4 yr

shelf life

-

shelf life

-

shelf life

-

purified by

using Protein A

purified by

affinity chromatography (affinity tag purification)

purified by

-

purified by

-

General description

Monoclonal ANTI-FLAG M2 is a purified immunoglobulin, IgG1, monoclonal antibody, purified from culture supernatant of hybridoma cells, that binds to FLAG® fusion proteins. Unlike ANTI-FLAG M1 antibody, the M2 antibody will recognize the FLAG sequence at the N-terminus, Met-N-terminus, C-terminus, or at an internal site of FLAG fusion proteins. Monoclonal ANTI-FLAG M2 is useful for identification and capture of FLAG fusion proteins by common immunological procedures such as Western blots and immunoprecipitation. It is also useful for affinity purification of FLAG fusion proteins when bound to a solid support.

form: solution pH 7.4, containing 15 mM sodium azide
concentration: 3.0-5.0 mg/mL

Application

IB, IF, IP, FACS, ELISA
Antibody is recommended for use in several applications such as immunoblotting, immunoprecipitation, immunofluorescence, flow cytometry, and ELISA.

Packaging

polypropylene screw cap vial

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in specified buffer

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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