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B3150

Sigma-Aldrich

Monoclonal Anti-Protein A−Biotin antibody produced in mouse

clone SPA-27, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-Protein A
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

SPA-27, monoclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:80,000-1:160,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Protein A is a cell wall associated protein isolated from Staphylococcus aureus Cowan 1 strain. The 42 kDa protein consists of a single polypeptide chain. Protein A possesses a specific affinity for Fc region of many mammalian immunoglobulins as human, rabbit, and guinea pig IgGs and therefore, considered as a universal reagent in biochemistry and immunology. Antibodies to protein A have been produced in rabbits, guinea pigs, chickens and mice. Protein A affinity chromatography has been achieved for the purification of immmunoglobulins. Monoclonal Anti-Protein A (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Immunogen

protein A from Cowan I strain of Staphy­lococcus aureus.

Application

Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Biochem/physiol Actions

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.

Storage and Stability

Store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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