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B9183

Sigma-Aldrich

Anti-HA-Biotin antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

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Synonym(s):
Monoclonal Anti-HA, Monoclonal Anti-HA-Biotin antibody produced in mouse, Anti-HA, Anti-Influenza Hemagglutinin
MDL number:
NACRES:
NA.56

biological source

mouse

Quality Level

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

HA-7, monoclonal

form

buffered aqueous solution

concentration

~1 mg/mL

technique(s)

western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.

shipped in

dry ice

storage temp.

−20°C
2-8°C

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This Item
H3663A5477B7405
vibrant-m

H3663

Anti-HA antibody, Mouse monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

conjugate

biotin conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

conjugate

biotin conjugate

form

buffered aqueous solution

form

-

form

buffered aqueous glycerol solution

form

buffered aqueous solution

storage temp.

−20°C, 2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

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General description

The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Antibody suitable for immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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