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C-12217

Sigma-Aldrich

Human Dermal Lymphatic Endothelial Cells (HDLEC)

Adult, 500,000 cryopreserved cells

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Synonym(s):
HDLEC cells

biological source

human dermis

packaging

pkg of 500,000 cells

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12216C-14021C-12225
packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

morphology

( endothelial)

morphology

( endothelial)

morphology

(endothelial)

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Skin

Application

Primary Human Dermal Lymphatic Endothelial Cells (HDLEC) are a subpopulation of the Human Dermal Endothelial Cells. They are isolated from the dermis of adult skin (different locations) from a single donor and are provided in a cryopreserved format. (Human Dermal Blood Endothelial Cells (HDBEC) from the same donor are available on request.)The cells are routinely analyzed by flow cytometric analysis and immunofluorescent staining: >95% of the cells are CD31 positive and podoplanin positive. In addition, the cells stain positive for Prox-1.Lymphatic vessels transport excess fluids from tissues to the circulatory system and are a major component of the immune system. The transported fluid is nearly cell free. The vessels are highly permeable, because they lack a continuous basal membrane. Lymphatic endothelial cells are involved in pathological alterations of the lymphatic system. During tumor lymphangiogenesis, the lymphatic endothelial cells build new vessels that infiltrate tumors, attract tumor cells, and induce tumor cell metastasis.

Quality

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: Podoplanin positive, CD31 positiveGuaranteed population doublings: >15

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Xinguo Jiang et al.
The Journal of clinical investigation, 130(10), 5562-5575 (2020-07-17)
Pathologic lymphatic remodeling in lymphedema evolves during periods of tissue inflammation and hypoxia through poorly defined processes. In human and mouse lymphedema, there is a significant increase of hypoxia inducible factor 1 α (HIF-1α), but a reduction of HIF-2α protein
Silvia Martin-Almedina et al.
The Journal of clinical investigation, 126(8), 3080-3088 (2016-07-12)
Hydrops fetalis describes fluid accumulation in at least 2 fetal compartments, including abdominal cavities, pleura, and pericardium, or in body tissue. The majority of hydrops fetalis cases are nonimmune conditions that present with generalized edema of the fetus, and approximately

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