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C-12644

Sigma-Aldrich

Human Tracheal Epithelial Cells (HTEpC)

500,000 cryopreserved cells

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biological source

human trachea

packaging

pkg of 500,000 cells

morphology

(epithelial)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

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This Item
C-14064C-14063C-14062
packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

morphology

(epithelial)

morphology

(epithelial)

morphology

(epithelial)

morphology

(epithelial)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Trachea

Application

Primary Human Tracheal Epithelial Cells (HTEpC) are isolated from the surface epithelium of human trachea and are provided in a cryopreserved format. They stain positive for cytokeratin.The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HTEpC are useful for investigating the function and pathology of the respiratory system.

Quality

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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