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C-14031

Sigma-Aldrich

Normal Human Dermal Fibroblasts (NHDF)

Adult, 1 million cells per pellet

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biological source

human dermis (adult)

packaging

pkg of 1,000,000 cells

morphology

(fibroblast)

application(s)

cell analysis (DNA, RNA, and protein)

shipped in

dry ice

storage temp.

−20°C

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This Item
C-12300C-14030C-12302
packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 500,000 cells

morphology

(fibroblast)

morphology

(fibroblast)

morphology

(fibroblast)

morphology

(fibroblast)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−196°C

storage temp.

−20°C

storage temp.

−196°C

application(s)

cell analysis (DNA, RNA, and protein)

application(s)

-

application(s)

cell analysis (DNA, RNA, and protein)

application(s)

-

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Skin

Application

Primary Normal Human Dermal Fibroblasts (NHDF) are isolated form the dermis of adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They can be used for wound healing studies and dermatological research to investigate diseases like scleroderma, fibrosarcoma, fibrosis, xeroderma pigmentosum, and histiocytoma. Moreover, fibroblasts are important for cancer research, tissue regeneration, and tissue engineering studies.Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.

Quality

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Physical form

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Subculture Routine

Click here for more information.

Other Notes

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Legal Information

RNAlater is a registered trademark of Ambion, Inc.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Primary Cell Culture Basics

What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

PromoCell® Primary Cell Pellets

Primary cells from PromoCell are available in pelleted form for research applications like gene expression analysis, and cell/protein characterization experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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