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C-39415

Sigma-Aldrich

Melanocyte Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

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Synonym(s):
Recommended with C-24210

form

aqueous solution

packaging

pkg of 1 vial

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

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This Item
C-39410C-24210C-24215
vibrant-m

C-39415

Melanocyte Growth Medium

vibrant-m

C-39410

Melanocyte Growth Medium

vibrant-m

C-24210

Melanocyte Growth Medium

vibrant-m

C-24215

Melanocyte Growth Medium

form

aqueous solution

form

liquid

form

aqueous solution

form

aqueous solution

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

packaging

pkg of 1 vial

packaging

pkg of 1 kit

packaging

pkg of 500 ml

packaging

pkg of 500 ml

General description

Melanocyte Growth Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-24210

Application

Melanocyte Growth Medium is a serum-free, PMA (Phorbol Myristate Acetate)-containing Medium and has been developed for the in vitro cultivation of juvenile melanocytes. Since PMA is a tumor promoting mitogen, it may interfere with some experimental approaches. In these cases, we recommend using the PMA-free Melanocyte Growth Medium M2 (C-24300). The Melanocyte Growth Medium is optimized for primary human cells, but can also be used for equine, porcine, murine, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Melanocyte Growth Medium are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Bovine Pituitary Extract: 0.004 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 0.5 µg / mlPhorbol Myristate Acetate: 10 ng / ml

Storage and Stability

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subculture Routine

Click here for more information.

recommended

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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