MilliporeSigma
All Photos(2)

Documents

C5878

Sigma-Aldrich

Chlorophyll b from spinach

≥90% (HPLC), ≤0.5% Chlorophyll a

Sign Into View Organizational & Contract Pricing

Empirical Formula (Hill Notation):
C55H70MgN4O6
CAS Number:
Molecular Weight:
907.47
Beilstein:
4122778
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Assay

≥90% (HPLC)

form

powder

impurities

≤0.5% Chlorophyll a

mp

183-185 °C (lit.)

ε (extinction coefficient)

15.9 × 104 at 452 nm in diethyl ether (lit.)
5.61 × 104 at 644 nm in diethyl ether (lit.)

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

shipped in

dry ice

storage temp.

−20°C

SMILES string

[H]C(=O)c1c(CC)c2cc3c(C)c4C(=O)[C@H](C(=O)OC)c5c6nc(cc7c(C)c(C=C)c(cc1n2)n7[Mg]n3c45)[C@@H](C)[C@@H]6CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C

InChI

1S/C55H71N4O6.Mg/c1-12-38-35(8)42-27-43-36(9)40(23-24-48(61)65-26-25-34(7)22-16-21-33(6)20-15-19-32(5)18-14-17-31(3)4)52(58-43)50-51(55(63)64-11)54(62)49-37(10)44(59-53(49)50)28-46-39(13-2)41(30-60)47(57-46)29-45(38)56-42;/h12,25,27-33,36,40,51H,1,13-24,26H2,2-11H3,(H-,56,57,58,59,60,62);/q-1;+2/p-1/b34-25+;/t32-,33-,36+,40+,51-;/m1./s1

InChI key

NSMUHPMZFPKNMZ-VBYMZDBQSA-M

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C5753F3013F0628
Chlorophyll a from spinach

Sigma-Aldrich

C5753

Chlorophyll a

form

powder

form

powder

form

lyophilized powder

form

lyophilized powder

impurities

≤0.5% Chlorophyll a

impurities

chlorophyll b, essentially free

impurities

-

impurities

-

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

application(s)

-

application(s)

cell analysis

application(s)

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Chlorophyll b is one of the chlorophyll pigments that impart leaves their green color. Chlorophyll is a Greek name with chlorós meaning green and phyllon meaning leaf. The structure of Chlorophyll b was successfully decoded by Fischer and Wenderoth. It is present in peripheral antenna complexes along with chlorophyll a. The amount of chlorophyll b in spinach is found to be lesser than chlorophyll a.

Application

Chlorophyll b from spinach has been used:
  • to perform quantitative analysis of the components of carotenoid and chlorophyll in the monovarietal virgin olive oil samples
  • to study the function of chlorophyll and porphyrins in the photo-oxidation of dairy products using sensory evaluation and fluorescence spectroscopy
  • to analyze the limits of application of spectrophotometry and spectrofluorimetry in the determination of Chlorophyll a/b ratio
  • to examine the changes in chlorophyll -a & -b content and color of grapevine leaves

Biochem/physiol Actions

Chlorophyll b undergoes breakdown to form pheophytin b by the enzyme chlorophyllase. This causes a change in its color from green to olive-brown. It is involved in the harvesting of light energy during the process of photosynthesis and its subsequent conversion to chemical energy. Along with chlorophyll a, it plays a key role in the ability of a plant to adapt to different light intensities.
Chlorophyll b extends the absorption spectrum of light-harvesting complexes LHC-I and LHC-II above 700 nm, a region of the visible spectrum that is not absorbed when only chlorophyll a is present in the LHCs.

Packaging

Sealed ampule.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

β-Carotene synthetic, ≥93% (UV), powder

Sigma-Aldrich

C9750

β-Carotene

Violaxanthin ≥90.0% (HPLC)

Sigma-Aldrich

52444

Violaxanthin

Copper Chlorophyllin Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1461

Copper Chlorophyllin

The role of naturally occurring chlorophyll and porphyrins in light-induced oxidation of dairy products. A study based on fluorescence spectroscopy and sensory analysis
Wold JP, et al.
International dairy journal, 15(4), 343-353 (2005)
Chlorophyll and colour changes in grapevine leaves preserved by passive modification
K?rca A, et al.
European Food Research and Technology, 223(3), 387-393 (2006)
On the limits of applicability of spectrophotometric and spectrofluorimetric methods for the determination of chlorophyll a/b ratio
Kouril R, et al.
Photosynthesis Research, 62(1), 107-116 (1999)
A Tanaka et al.
Proceedings of the National Academy of Sciences of the United States of America, 95(21), 12719-12723 (1998-10-15)
Chlorophyll b is an ubiquitous accessory pigment in land plants, green algae, and prochlorophytes. Its biosynthesis plays a key role in the adaptation to various light environments. We isolated six chlorophyll b-less mutants by insertional mutagenesis by using the nitrate
A Ruiz-Martinez et al.
Bioresource technology, 126, 247-253 (2012-10-18)
This study investigated the removal of nitrogen and phosphorus from the effluent of a submerged anaerobic membrane bioreactor (SAnMBR) by means of a lab-scale photobioreactor in which algae biomass was cultured in a semi-continuous mode for a period of 42

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service