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C8409

Sigma-Aldrich

Anti-Cortisol antibody produced in rabbit

whole antiserum, lyophilized powder

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Synonym(s):
Anti-Cortisol Antibody, Cortisol Antibody, Cortisol Antibody - Anti-Cortisol antibody produced in rabbit, Anti-Hydrocortisone
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

radioimmunoassay: suitable (dextran coated charcoal 3H RIA)

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
C8784S6390G9152
antibody form

whole antiserum

antibody form

whole antiserum

antibody form

whole antiserum

antibody form

whole antiserum

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

technique(s)

radioimmunoassay: suitable (dextran coated charcoal 3H RIA)

technique(s)

radioimmunoassay: suitable (dextran coated charcoal 3H RIA)

technique(s)

immunoelectrophoresis: suitable, indirect ELISA: 1:10,000

technique(s)

dot blot: 1:50 using galactocerebroside I and II

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General description

Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD).

Immunogen

cortisol-21-thyroglobulin.

Application

Anti-Cortisol antibody has been used in Plasma hormone assay and immunofluorescence.
Anti-cortisol antibody is suitable for RIA. It has been used for ELISA to quantitate the levels of cortisol in the blood of fish. The antibody was also used for immunohistochemistry of trout gill filament sections at a working dilution of 1:5.

Biochem/physiol Actions

Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD). Cortisol is bound to corticosteroid-binding globulin in the plasma. The organs that produce 11-HSD and involved in metabolizing and clearing it from plasma cortisol are kidney and the liver. Cortisol may be oxidised at 11-hydroxt position to result in the production of cortisone, dihydrocortisone, or tetrahydrocortisone. Cortisol and other glucocorticoids bind to the human glucocorticoid receptor (hGR) that is predominantly present in the cytoplasm. On binding, the recptor-ligand complex translocate to nucleus where the transcription of target genes is induced. Deficiency in the levels of 11-HSD are responsible for juvenile hypertension, (a feature of Apparent Mineralocorticoid Excess syndrome), edema, heart failure and hypokalemia (licorice intoxication).

Reconstitution

Reconstitute with 5 mL buffer: 0.05 M Tris-HCl, pH 8.0, 0.1 M sodium chloride, 0.1% gelatin, and 15 mM sodium azide. Store this stock at −20 °C. Working dilution (1:10) to be discarded if unused within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Kamilia Henna et al.
Veterinary world, 14(9), 2419-2426 (2021-11-30)
Goats are widely distributed in southwest Algeria. The Saharan goat is perfectly adapted to the harsh conditions of arid areas, and it is characterized by resistance to long photoperiod and reduced metabolic needs, allowing the survival of its offspring by
Qiqi Yang et al.
Scientific reports, 7(1), 10315-10315 (2017-09-06)
Hepatocellular carcinoma (HCC) is more prevalent in men than in women. Previously we have found that some stromal cells, including hepatic stellate cells (HSCs), neutrophils and macrophages, play crucial roles in promoting sex disparity in kras
Activation of liver stromal cells is associated with male-biased liver tumor initiation in xmrk and Myc transgenic zebrafish
Yang Q, et al.
Scientific Reports, 7(1), 10315-10315 (2017)
Development of a microtitre plate indirect ELISA for measuring cortisol in teleosts, and evaluation of stress responses in rainbow trout and gilthead sea bream
Tintos A et al
Journal of Fish Biology, 68, 251-263 (2006)
P Laurent et al.
Fish physiology and biochemistry, 13(5), 353-370 (1994-11-01)
The aim of this work was to determine the kinetics of the dramatic development of the gill chloride cells (CCs) during adaptation of the salmonid Oncorhynchus mykiss to an ion-poor environment.To monitor cell division, the incorporation in the mitotic cell

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