C8409
Anti-Cortisol antibody produced in rabbit
whole antiserum, lyophilized powder
Synonym(s):
Anti-Cortisol Antibody, Cortisol Antibody, Cortisol Antibody - Anti-Cortisol antibody produced in rabbit, Anti-Hydrocortisone
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About This Item
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized powder
technique(s)
radioimmunoassay: suitable (dextran coated charcoal 3H RIA)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD).
Immunogen
cortisol-21-thyroglobulin.
Application
Anti-Cortisol antibody has been used in Plasma hormone assay and immunofluorescence.
Anti-cortisol antibody is suitable for RIA. It has been used for ELISA to quantitate the levels of cortisol in the blood of fish. The antibody was also used for immunohistochemistry of trout gill filament sections at a working dilution of 1:5.
Biochem/physiol Actions
Cortisol is the principal secretary glucocorticoid of human adrenal cortex. It is produced as cortisone but converted to a biologically active form, cortisol, by the activity of enzyme 11β- hydroxysteroid dehydrogenase (11-HSD). Cortisol is bound to corticosteroid-binding globulin in the plasma. The organs that produce 11-HSD and involved in metabolizing and clearing it from plasma cortisol are kidney and the liver. Cortisol may be oxidised at 11-hydroxt position to result in the production of cortisone, dihydrocortisone, or tetrahydrocortisone. Cortisol and other glucocorticoids bind to the human glucocorticoid receptor (hGR) that is predominantly present in the cytoplasm. On binding, the recptor-ligand complex translocate to nucleus where the transcription of target genes is induced. Deficiency in the levels of 11-HSD are responsible for juvenile hypertension, (a feature of Apparent Mineralocorticoid Excess syndrome), edema, heart failure and hypokalemia (licorice intoxication).
Reconstitution
Reconstitute with 5 mL buffer: 0.05 M Tris-HCl, pH 8.0, 0.1 M sodium chloride, 0.1% gelatin, and 15 mM sodium azide. Store this stock at −20 °C. Working dilution (1:10) to be discarded if unused within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Mercer J G, et al.
Physiology & Behavior, 60(1), 121-127 (1996)
Licorice-induced pseudoaldosteronism, hypertension, hypokalemia, aldosteronopenia, and suppressed plasma renin activity
Conn JW et al
Journal of the American Medical Association, 205, 492-496 (1968)
Unusual steroid excretion in a child with low-renin hypertension
Werder EA et al
Research on Steroids, 6, 385-389 (1974)
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