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biological source

fish caudal trunk

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


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Cell Line Origin

Fish bluegill fry

Cell Line Description

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings (Bluegill fry, Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV).


Fish virus studies

Culture Medium

EMEM(HBSS) (M5775) or EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 850 mg/L Sodium Bicarbonate(NaHCO3) (S8761) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442); a mixture of MEM (HBSS) and MEM (EBSS) with 1-2% FBS is best for stability of monolayer and maintenance medium

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15 - 33°C, optimal temperature is 23 - 26°C; monolayer is stable for at least 10 days in maintenance medium (at 15 or 16°C for about 21 days). Fish c

Other Notes

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