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Key Documents

CHSE-214

21714, fish embryo, Fibroblast-like

Synonym(s):

CHSE/F

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About This Item

UNSPSC Code:
41106514

product name

CHSE-214, 00021714

biological source

fish embryo

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Common bluegill (Lepomis macrochirus), normal

Cell Line Description

The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.

Application

Fish virus studies

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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